Multigene phylogenetics reveals temporal diversification of major African malaria vectors.
Multigene phylogenetics reveals temporal diversification of major African malaria vectors.
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DOI：
10.1371/journal.pone.0093580
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Sharakhov IV
中科院分区：
文献类型：
--
作者：
Kamali M;Marek PE;Peery A;Antonio-Nkondjio C;Ndo C;Tu Z;Simard F;Sharakhov IV
撒哈拉以南非洲疟疾的主要传播媒介属于塞利亚亚属。然而，非洲蚊子物种之间的系统发育关系和时间多样性尚未明确确定。有关载体进化历史的知识对于正确解释通过比较基因组学分析确定的遗传变化至关重要。在这项研究中，我们使用了49个非洲疟疾媒介的基因序列来估计分子系统发育。冈比亚亚纲，An.Funestus，An.尼丽，亚洲疟疾蚊子。Stephensi，外群种致倦库蚊和埃及伊蚊。为了推测系统发育，我们鉴定了大约每5Mb均匀分布在五条染色体臂上的同源序列。对序列进行比对，并用最大似然法和邻接法推断系统发育树。使用放松对数正态模型的贝叶斯分子测年被用来推断发散时间。来自单个基因的树彼此一致，放置了一个。尼利作为一个基本支系，从4760万年前所研究的疟疾蚊子物种(Mya)分化而来。其他非洲疟疾媒介起源较晚，并独立获得了与媒介能力有关的特征。这种血统导致了一个。冈比亚按蚊分流30.4万，而非洲按蚊和按蚊传播。Funestus和亚洲的载体An。Stephensi是最接近的姐妹类群，分裂了20.8mya。这些结果得到了为每条染色体臂单独生成的连锁系统发育树中持续较高的自助值的支持。全基因组多基因系统发育分析是识别疟疾媒介之间历史关系的有用方法，为正确解释跨物种的基因组变化提供了一个框架，并理解了这种普遍存在的致命昆虫传播疾病的进化起源。
The major vectors of malaria in sub-Saharan Africa belong to subgenus Cellia. Yet, phylogenetic relationships and temporal diversification among African mosquito species have not been unambiguously determined. Knowledge about vector evolutionary history is crucial for correct interpretation of genetic changes identified through comparative genomics analyses. In this study, we estimated a molecular phylogeny using 49 gene sequences for the African malaria vectors An. gambiae, An. funestus, An. nili, the Asian malaria mosquito An. stephensi, and the outgroup species Culex quinquefasciatus and Aedes aegypti. To infer the phylogeny, we identified orthologous sequences uniformly distributed approximately every 5 Mb in the five chromosomal arms. The sequences were aligned and the phylogenetic trees were inferred using maximum likelihood and neighbor-joining methods. Bayesian molecular dating using a relaxed log normal model was used to infer divergence times. Trees from individual genes agreed with each other, placing An. nili as a basal clade that diversified from the studied malaria mosquito species 47.6 million years ago (mya). Other African malaria vectors originated more recently, and independently acquired traits related to vectorial capacity. The lineage leading to An. gambiae diverged 30.4 mya, while the African vector An. funestus and the Asian vector An. stephensi were the most closely related sister taxa that split 20.8 mya. These results were supported by consistently high bootstrap values in concatenated phylogenetic trees generated individually for each chromosomal arm. Genome-wide multigene phylogenetic analysis is a useful approach for discerning historic relationships among malaria vectors, providing a framework for the correct interpretation of genomic changes across species, and comprehending the evolutionary origins of this ubiquitous and deadly insect-borne disease.
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影响因子：
3.8
作者：
della Torre, A;Tu, ZJ;Petrarca, V
通讯作者：
Petrarca, V
影响因子：
2.1
作者：
Awono-Ambene, HP;Kengne, P;Fontenille, D
通讯作者：
Fontenille, D
影响因子：
56.9
作者：
Coluzzi, M;Sabatini, A;Petrarca, V
通讯作者：
Petrarca, V
DOI：
10.1126/science.1191864
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Arensburger P;Megy K;Waterhouse RM;Abrudan J;Amedeo P;Antelo B;Bartholomay L;Bidwell S;Caler E;Camara F;Campbell CL;Campbell KS;Casola C;Castro MT;Chandramouliswaran I;Chapman SB;Christley S;Costas J;Eisenstadt E;Feschotte C;Fraser-Liggett C;Guigo R;Haas B;Hammond M;Hansson BS;Hemingway J;Hill SR;Howarth C;Ignell R;Kennedy RC;Kodira CD;Lobo NF;Mao C;Mayhew G;Michel K;Mori A;Liu N;Naveira H;Nene V;Nguyen N;Pearson MD;Pritham EJ;Puiu D;Qi Y;Ranson H;Ribeiro JM;Roberston HM;Severson DW;Shumway M;Stanke M;Strausberg RL;Sun C;Sutton G;Tu ZJ;Tubio JM;Unger MF;Vanlandingham DL;Vilella AJ;White O;White JR;Wondji CS;Wortman J;Zdobnov EM;Birren B;Christensen BM;Collins FH;Cornel A;Dimopoulos G;Hannick LI;Higgs S;Lanzaro GC;Lawson D;Lee NH;Muskavitch MA;Raikhel AS;Atkinson PW
通讯作者：
Atkinson PW
中国按蚊群（双翅目：蚊科）：核糖体 DNA 内转录间隔区 2 序列推断的物种区分和系统发育关系
影响因子：
2.1
作者：
Afrane, YA;Lawson, BW;Yan, GY
通讯作者：
Yan, GY