Increased expression of toll-like receptors 2 and 9 is associated with reduced DNA methylation in spontaneous preterm labor.
Increased expression of toll-like receptors 2 and 9 is associated with reduced DNA methylation in spontaneous preterm labor.
复制标题
DOI：
10.1016/j.jri.2017.05.003
复制
发表时间：
2017-06
影响因子：
3.4
通讯作者：
Strauss JF 3rd
中科院分区：
文献类型：
--
作者：
Walsh SW;Chumble AA;Washington SL;Archer KJ;Sahingur SE;Strauss JF 3rd
自发性早产（sPTL）的原因尚不清楚，但可能是由于表观遗传改变增加了蜕膜组织对炎症刺激的敏感性。我们收集了来自足月未分娩（TNL）、足月分娩（TL）和sPTL妇女的蜕膜组织。Illumina Infinium HumanMethylation 450 BeadChip分析显示，与TL相比，sPTL中TLR-2和TLR-9的DNA甲基化显著降低。免疫组织化学染色显示，与TL或TNL相比，sPTL妇女蜕膜组织中TLR-2和TLR-9的表达显著增加。TLR表达不存在于蜕膜细胞中，但定位于组织白细胞，如CD14、巨噬细胞抗原和中性粒细胞弹性蛋白酶染色所示。炎症基因的微阵列分析，以评估先天性免疫反应表明，显着增加表达的炎症细胞因子和趋化因子与TL的妇女相比，TNL。然而，当sPTL与TL相比时，炎性细胞因子进一步增加，中性粒细胞趋化因子显著增加。这些结果表明，表观遗传机制可能在增加白细胞浸润和增加蜕膜组织对炎症刺激的敏感性方面发挥作用，这些炎症刺激可能会导致早产。
The cause of spontaneous preterm labor (sPTL) is not known, but it could be due to epigenetic alterations that increase the sensitivity of decidual tissue to inflammatory stimuli. We collected decidual tissue from women at term not in labor (TNL), women at term in labor (TL), and women with sPTL. Illumina Infinium HumanMethylation450 BeadChip analysis revealed significantly reduced DNA methylation for TLR-2 and TLR-9 in sPTL as compared to TL. Immunohistochemical staining documented significantly increased expression of TLR-2 and TLR-9 in decidual tissue of women with sPTL as compared to TL or TNL. TLR expression was not present in decidual cells, but localized to tissue leukocytes as revealed by staining for CD14, a macrophage antigen, and neutrophil elastase. Microarray analysis of inflammatory genes to assess innate immune response demonstrated marked increases in expression of inflammatory cytokines and chemokines in women with TL as compared to TNL. However, when sPTL was compared to TL, there was a further increase in inflammatory cytokines, and a remarkable increase in neutrophil chemokines. These results suggest that epigenetic mechanisms could play a role in increasing leukocyte infiltration, and increasing the sensitivity of decidual tissue to inflammatory stimuli that could precipitate labor prematurely.
登录
查看更多内容
影响因子：
7.4
作者：
Gill, Roop;Tsung, Allan;Billiar, Timothy
通讯作者：
Billiar, Timothy
DOI：
10.1017/s2040174412000104
发表时间：
2012-06
影响因子：
1.7
作者：
Burris, H. H.;Rifas-Shiman, S. L.;Baccarelli, A.;Tarantini, L.;Boeke, C. E.;Kleinman, K.;Litonjua, A. A.;Rich-Edwards, J. W.;Gillman, M. W.
通讯作者：
Gillman, M. W.
影响因子：
2.9
作者：
Hsieh, T'sang-T'ang;Chen, Szu-Fu;Hung, Tai-Ho
通讯作者：
Hung, Tai-Ho
影响因子：
3.7
作者：
Aschbacher, Kirstin;O'Donovan, Aoife;Wolkowitz, Owen M.;Dhabhar, Firdaus S.;Su, Yali;Epel, Elissa
通讯作者：
Epel, Elissa
影响因子：
3.7
作者：
Castro-Leyva V;Espejel-Nuñez A;Barroso G;Zaga-Clavellina V;Flores-Pliego A;Morales-Mendez I;Giono-Cerezo S;Walsh SW;Estrada-Gutierrez G
通讯作者：
Estrada-Gutierrez G