Downregulation of miRNA-200c links breast cancer stem cells with normal stem cells.
Downregulation of miRNA-200c links breast cancer stem cells with normal stem cells.
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DOI：
10.1016/j.cell.2009.07.011
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发表时间：
2009-08-07
期刊：
影响因子：
64.5
通讯作者：
Clarke MF
中科院分区：
文献类型：
--
作者：
Shimono Y;Zabala M;Cho RW;Lobo N;Dalerba P;Qian D;Diehn M;Liu H;Panula SP;Chiao E;Dirbas FM;Somlo G;Pera RA;Lao K;Clarke MF
人类乳腺肿瘤含有乳腺癌干细胞（BCSC）群体，其性质令人想起正常干细胞。我们发现了37种在人BCSC和非致瘤性癌细胞之间差异表达的microRNA。三个簇，miR-200 c-141，miR-200 b-200 a-429和miR-183-96-182在人BCSC，正常人和小鼠乳腺干/祖细胞和胚胎癌细胞中下调。miR-200 c调节干细胞自我更新的已知调节因子BMI 1的表达。MiR-200 c在体外抑制乳腺癌细胞的克隆形成，并抑制胚胎癌细胞的生长。最重要的是，miR-200 c强烈抑制正常乳腺干细胞形成乳腺导管的能力和体内由人BCSC驱动的肿瘤形成。三个microRNA簇的协同下调和miR-200 c对克隆形成的类似功能调节提供了将乳腺癌干细胞与正常干细胞连接起来的分子联系。
Human breast tumors contain a breast cancer stem cell (BCSC) population with properties reminiscent of normal stem cells. We found 37 microRNAs that were differentially expressed between human BCSCs and non-tumorigenic cancer cells. Three clusters, miR-200c-141, miR-200b-200a-429 and miR-183-96-182 were down-regulated in human BCSCs, normal human and murine mammary stem/progenitor cells and embryonal carcinoma cells. Expression of BMI1, a known regulator of stem cell self-renewal, was modulated by miR-200c. MiR-200c inhibited the clonogenicity of breast cancer cells and suppressed the growth of embryonal carcinoma cells in vitro. Most importantly, miR-200c strongly suppressed the ability of normal mammary stem cells to form mammary ducts and tumor formation driven by human BCSCs in vivo. The coordinated down-regulation of three microRNA clusters and the similar functional regulation of clonogenicity by miR-200c provide a molecular link that connects breast cancer stem cells with normal stem cells.
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影响因子：
4.5
作者：
Christoffersen, Nanna Ronbjerg;Silahtaroglu, Asli;Lund, Anders H.
通讯作者：
Lund, Anders H.
影响因子：
5.3
作者：
Davis, Tigwa H.;Cuellar, Trinna L.;Ullian, Erik M.
通讯作者：
Ullian, Erik M.
DOI：
10.1073/pnas.0703478104
发表时间：
2007-06-12
影响因子：
11.1
作者：
Dalerba, Piero;Dylla, Scott J.;Clarke, Michael F.
通讯作者：
Clarke, Michael F.
影响因子：
11.2
作者：
Daniel VC;Marchionni L;Hierman JS;Rhodes JT;Devereux WL;Rudin CM;Yung R;Parmigiani G;Dorsch M;Peacock CD;Watkins DN
通讯作者：
Watkins DN
影响因子：
11.2
作者：
Iorio, MV;Ferracin, M;Croce, CM
通讯作者：
Croce, CM