cAMP Response Element Binding Protein Expression in the Hippocampus of Rhesus Macaques with Chronic Ephedrine Addiction.
cAMP Response Element Binding Protein Expression in the Hippocampus of Rhesus Macaques with Chronic Ephedrine Addiction.
复制标题
慢性麻黄碱成瘾恒河猴海马cAMP反应元件结合蛋白表达
DOI：
10.1155/2017/1931204
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发表时间：
2017
影响因子：
--
通讯作者：
Ma S
中科院分区：
文献类型：
--
作者：
Sun Z;Ma Y;Duan S;Xie L;Lv J;Huang J;Lin Z;Guo R;Ma S
背景 药物成瘾被归类为一种具有复杂神经生物学机制的慢性复发性脑部疾病。越来越多的研究人员正在研究解决这一棘手问题的可能机制。方法建立恒河猴麻黄碱慢性成瘾模型，监测其体重和行为的变化。通过实时PCR和Western blot检测恒河猴海马cAMP反应元件结合蛋白(CREB)的表达。结果成功建立恒河猴慢性麻黄碱成瘾模型。他们表现出体重和行为的变化。免疫荧光显示海马细胞核中有CREB表达，实时荧光定量PCR和Western blot检测海马CREB mRNA和蛋白表达均增加。模型组海马CREB阳性表达量显着高于对照组。结论麻黄碱慢性成瘾后恒河猴体重和行为学发生显着变化。麻黄碱慢性成瘾恒河猴海马CREB的变化是重要的分子机制，CREB的上调可能参与了慢性麻黄碱成瘾个体的生理病理和行为过程。
Background Drug addiction is classified as a chronic relapse nature brain disease with complicated neurobiology mechanisms. There are an increasing number of researchers that are investigating the possible mechanisms for solving the thorny problem. Methods The model of chronic addiction of rhesus monkey ephedrine was established, where changes in body weight and behavior were monitored. The expression of cAMP response element binding protein (CREB) in the hippocampus of rhesus monkeys was identified by real-time PCR and Western blot. Results We were successful in establishing the chronic ephedrine addiction model in the rhesus macaques. They exhibited changes in body weight and behavior. Immunofluorescence showed that CREB was expressed in the nucleus of the hippocampus, and the expression of CREB mRNA and protein in the hippocampus were increased by real-time PCR and Western blot. The CREB positive expression in the hippocampus of the modeling group was significantly higher than in the control group. Conclusions The changes of body weight and behavior of the rhesus monkeys after ephedrine chronic addiction were significant. The changes of CREB in the hippocampus of rhesus macaques with ephedrine chronic addiction are important molecular mechanisms, and the upregulation of CREB may be involved in the physiological pathology and behavior process in individuals with chronic ephedrine addiction.
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影响因子：
10.6
作者：
Gold, Mark S.;Kobeissy, Firas H.;Wang, Kevin K. W.;Merlo, Lisa J.;Bruijnzeel, Adriaan W.;Krasnova, Irina N.;Cadet, Jean Lud
通讯作者：
Cadet, Jean Lud
影响因子：
4.2
作者：
Anderson, KG;Winger, G;Woolverton, WL
通讯作者：
Woolverton, WL
影响因子：
3.6
作者：
Miller, DK;Segert, IL
通讯作者：
Segert, IL
影响因子：
2.7
作者：
Pittenger ST;Barrett ST;Chou S;Bevins RA
通讯作者：
Bevins RA
影响因子：
2.9
作者：
Taheri S;Xun Z;See RE;Joseph JE;Reichel CM
通讯作者：
Reichel CM