Histone H2A.Z cooperates with RNAi and heterochromatin factors to suppress antisense RNAs.
Histone H2A.Z cooperates with RNAi and heterochromatin factors to suppress antisense RNAs.
复制标题
组蛋白H2A.Z与RNAi和异染色质因子合作以抑制反义RNA。
作者：
Eukaryotic transcriptomes are characterized by widespread transcription of non-coding and antisense RNAs, which is linked to key chromosomal processes, such as chromatin remodeling, gene regulation, and heterochromatin assembly. However, these transcripts can be deleterious, and their accumulation is suppressed by several mechanisms including degradation by the nuclear exosome. The mechanisms by which cells differentiate coding RNAs from transcripts targeted for degradation are not clear. Here we show that the variant histone H2A.Z, which is loaded preferentially at the 5' ends of genes by the Swr1 complex containing a JmjC domain protein, mediates suppression of antisense transcripts in the fission yeast Schizosaccharomyces pombe genome. H2A.Z is partially redundant in this regard with the Clr4/Suv39h-containing heterochromatin silencing complex that is also distributed at euchromatic loci, and with RNAi component Argonaute (Ago1). Loss of Clr4 or Ago1 alone has little effect on antisense transcript levels, but cells lacking either of these factors and H2A.Z show markedly increased levels of antisense RNAs that are normally degraded by the exosome. These analyses suggest that in addition to performing other functions, H2A.Z is a component of a genome indexing mechanism that cooperates with heterochromatin and RNAi factors to suppress read-through antisense transcripts.
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影响因子：
64.5
作者：
Cawley, S;Bekiranov, S;Gingeras, TR
通讯作者：
Gingeras, TR
影响因子：
16.8
作者：
Nicolas, Estelle;Yamada, Takatomi;Grewal, Shiv I. S.
通讯作者：
Grewal, Shiv I. S.
DOI：
10.1126/science.1162609
发表时间：
2008-10-17
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Roguev A;Bandyopadhyay S;Zofall M;Zhang K;Fischer T;Collins SR;Qu H;Shales M;Park HO;Hayles J;Hoe KL;Kim DU;Ideker T;Grewal SI;Weissman JS;Krogan NJ
通讯作者：
Krogan NJ
DOI：
10.1007/bf00282226
发表时间：
1994-12-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
CARR, AM;DORRINGTON, SM;NURSE, P
通讯作者：
NURSE, P
影响因子：
16.8
作者：
Zhang, Ke;Mosch, Kerstin;Grewal, Shiv I. S.
通讯作者：
Grewal, Shiv I. S.