Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer.
Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer.
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DOI：
10.1039/d2ay01197b
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发表时间：
2023-03-30
影响因子：
3.1
通讯作者：
Stone, Nick
中科院分区：
文献类型：
--
作者：
Bouzy, Pascaline;Lyburn, Iain D.;Pinder, Sarah E.;Scott, Robert;Mansfield, Jessica;Moger, Julian;Greenwood, Charlene;Bouybayoune, Ihssane;Cornford, Eleanor;Rogers, Keith;Stone, Nick
微钙化在癌症检测中起着重要作用。它们通过其放射学和组织学特征进行评估，但很难找到它们的形态，组成和特定类型乳腺病变性质之间的联系。虽然有一些乳房X线摄影的特点，要么是典型的良性或典型的恶性往往是不确定的外观。在这里，我们探索了大范围的振动光谱和多光子成像技术，以获得更多的信息的组成的微钙化。我们首次通过O-PTIR和拉曼光谱在同一时间、同一位置和同一高分辨率（0.5 μm）验证了微钙化中碳酸根离子的存在。此外，多光子成像的使用使我们能够创建模拟组织学图像的所有化学信息的受激拉曼组织学（SRH）图像。总之，我们建立了一个协议，有效地分析微钙化，通过迭代细化感兴趣的区域。我们的目标是开发一种协议，有效地分析乳腺微钙化和他们的微环境详细结合O-PTIR和拉曼光谱在同一时间，同一位置和相同的高分辨率（0.5 μm）。
Microcalcifications play an important role in cancer detection. They are evaluated by their radiological and histological characteristics but it is challenging to find a link between their morphology, their composition and the nature of a specific type of breast lesion. Whilst there are some mammographic features that are either typically benign or typically malignant often the appearances are indeterminate. Here, we explore a large range of vibrational spectroscopic and multiphoton imaging techniques in order to gain more information about the composition of the microcalcifications. For the first time, we validated the presence of carbonate ions in the microcalcifications by O-PTIR and Raman spectroscopy at the same time, the same location and the same high resolution (0.5 μm). Furthermore, the use of multiphoton imaging allowed us to create stimulated Raman histology (SRH) images which mimic histological images with all chemical information. In conclusion, we established a protocol for efficiently analysing the microcalcifications by iteratively refining the area of interest. We aim to develop a protocol for efficiently analysing breast microcalcifications and their microenvironment in detail by combining O-PTIR and Raman spectroscopy at the same time, the same location and the same high resolution (0.5 μm).
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DOI：
10.1017/s1551929520000917
发表时间：
2020-05
期刊：
Microscopy today
影响因子：
--
作者：
Kansiz M;Prater C;Dillon E;Lo M;Anderson J;Marcott C;Demissie A;Chen Y;Kunkel G
通讯作者：
Kunkel G
影响因子：
11.2
作者：
Barman I;Dingari NC;Saha A;McGee S;Galindo LH;Liu W;Plecha D;Klein N;Dasari RR;Fitzmaurice M
通讯作者：
Fitzmaurice M
影响因子：
--
作者：
Johnson, JM;Dalton, RR;Lambert, PJ
通讯作者：
Lambert, PJ
影响因子：
14
作者：
Antonakos, Anastasios;Liarokapis, Efthymios;Leventouri, Theodora
通讯作者：
Leventouri, Theodora
DOI：
10.1039/d1an01548f
发表时间：
2022-04-11
期刊：
The Analyst
影响因子：
--
作者：
Gosling S;Calabrese D;Nallala J;Greenwood C;Pinder S;King L;Marks J;Pinto D;Lynch T;Lyburn ID;Hwang ES;Grand Challenge Precision Consortium;Rogers K;Stone N
通讯作者：
Stone N