Flagellar biosynthesis exerts temporal regulation of secretion of specific Campylobacter jejuni colonization and virulence determinants.
Flagellar biosynthesis exerts temporal regulation of secretion of specific Campylobacter jejuni colonization and virulence determinants.
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DOI：
10.1111/mmi.12711
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发表时间：
2014-09
影响因子：
3.6
通讯作者：
Hendrixson DR
中科院分区：
文献类型：
--
作者：
Barrero-Tobon AM;Hendrixson DR
空肠弯曲杆菌鞭毛分别输出形成用于游泳运动性和定殖的鞭毛细胞器的蛋白质和促进禽类肠道定殖或人类肠细胞入侵的毒力因子。我们探讨了C.空肠鞭毛是一种多功能的分泌细胞器，通过检测允许定殖和毒力因子利用鞭毛进行自身分泌的分子决定簇。鞭毛生物发生观察到施加时间控制这些蛋白质的分泌，这表明在钩生物发生与细丝聚合本身减少这些因子的分泌过程中发生了大量的定植和毒力因子的分泌。此外，我们发现这些蛋白质的鞭毛依赖性分泌的分子内和分子间的要求是最令人联想到鞭毛蛋白分泌。重要的是，我们发现，一种定植和病毒因子Ciplatin的分泌不是人类结肠细胞入侵所必需的，这与先前关于这种蛋白质在入侵过程中如何发挥作用的假设相反。相反，C.空肠，以促进天然禽类宿主的肠道定殖。我们的工作提供了深入了解细菌鞭毛作为分泌机器的多功能性，可以输出促进不同生物过程的蛋白质。
The Campylobacter jejuni flagellum exports both proteins that form the flagellar organelle for swimming motility and colonization and virulence factors that promote commensal colonization of the avian intestinal tract or invasion of human intestinal cells, respectively. We explored how the C. jejuni flagellum is a versatile secretory organelle by examining molecular determinants that allow colonization and virulence factors to exploit the flagellum for their own secretion. Flagellar biogenesis was observed to exert temporal control of secretion of these proteins, indicating that a bolus of secretion of colonization and virulence factors occurs during hook biogenesis with filament polymerization itself reducing secretion of these factors. Furthermore, we found that intramolecular and intermolecular requirements for flagellar-dependent secretion of these proteins were most reminiscent to those for flagellin secretion. Importantly, we discovered that secretion of one colonization and virluence factor, CiaI, was not required for invasion of human colonic cells, which counters previous hypotheses for how this protein functions during invasion. Instead, secretion of CiaI was essential for C. jejuni to facilitate commensal colonization of the natural avian host. Our work provides insight into the versatility of the bacterial flagellum as a secretory machine that can export proteins promoting diverse biological processes.
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影响因子：
3.6
作者：
Christensen JE;Pacheco SA;Konkel ME
通讯作者：
Konkel ME
影响因子：
16
作者：
Birtalan, SC;Phillips, RM;Ghosh, P
通讯作者：
Ghosh, P
DOI：
10.1073/pnas.1113013108
发表时间：
2011-12-13
影响因子：
11.1
作者：
Boll, Joseph M.;Hendrixson, David R.
通讯作者：
Hendrixson, David R.
影响因子：
3.6
作者：
Fraser, GM;Bennett, JCQ;Hughes, C
通讯作者：
Hughes, C
影响因子：
6.4
作者：
Costa SC;Schmitz AM;Jahufar FF;Boyd JD;Cho MY;Glicksman MA;Lesser CF
通讯作者：
Lesser CF