Intrinsic histone-DNA interactions are not the major determinant of nucleosome positions in vivo.
Intrinsic histone-DNA interactions are not the major determinant of nucleosome positions in vivo.
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DOI：
10.1038/nsmb.1636
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发表时间：
2009-08
影响因子：
16.8
通讯作者：
中科院分区：
文献类型：
--
作者：
我们评估的作用，内在组蛋白-DNA相互作用的映射核小体组装在体外基因组DNA。核小体强烈偏好酵母DNA而不是E.大肠杆菌DNA，表明酵母基因组进化有利于核小体的形成。许多酵母启动子和终止子区域本质上不利于核小体的形成，并且体外组装的核小体显示出强的旋转定位。由ACF组装因子产生的核小体阵列显示更少的无核小体区域，减少旋转定位，和更少的翻译定位比通过内在组蛋白-DNA相互作用获得的。重要的是，体外组装的核小体仅显示对特定翻译位置的有限偏好，并且不显示体内观察到的模式。我们的研究结果反对核小体定位的基因组代码，他们认为，在编码区的核小体模式主要来自统计定位从附近的启动子，涉及RNA聚合酶II的转录起始的某些方面的障碍。
We assess the role of intrinsic histone-DNA interactions by mapping nucleosomes assembled in vitro on genomic DNA. Nucleosomes strongly prefer yeast DNA over E. coli DNA, indicating that the yeast genome evolved to favor nucleosome formation. Many yeast promoter and terminator regions intrinsically disfavor nucleosome formation, and nucleosomes assembled in vitro display strong rotational positioning. Nucleosome arrays generated by the ACF assembly factor display fewer nucleosome-free regions, reduced rotational positioning, and less translational positioning than obtained by intrinsic histone-DNA interactions. Importantly, in vitro assembled nucleosomes display only a limited preference for specific translational positions and do not show the pattern observed in vivo. Our results argue against a genomic code for nucleosome positioning, and they suggest that the nucleosomal pattern in coding regions arises primarily from statistical positioning from a barrier near the promoter that involves some aspect of transcriptional initiation by RNA polymerase II.
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