Regulation of recombination between gtfB/gtfC genes in Streptococcus mutans by recombinase A.
Regulation of recombination between gtfB/gtfC genes in Streptococcus mutans by recombinase A.
复制标题
DOI：
10.1155/2013/405075
复制
发表时间：
2013
影响因子：
--
通讯作者：
Matsumoto-Nakano M
中科院分区：
文献类型：
--
作者：
Inagaki S;Fujita K;Takashima Y;Nagayama K;Ardin AC;Matsumi Y;Matsumoto-Nakano M
变形链球菌产生三种葡萄糖基转移酶（GTFs），它们的协同作用对细胞粘附至关重要。重组酶A （RecA）蛋白是同源重组所必需的。在我们之前的研究中，我们分离了几株菌落形态光滑且GTF活性低的菌株，这些特征推测来自GTF融合。本研究的目的是探讨这些融合的机制。在重组RecA （rRecA）蛋白存在下培养S. mutans菌株MT8148，分离光滑菌落。测定该菌株及其他临床菌株gtfB和gtfC基因的生物学功能和序列。与MT8148相比，这些菌株的蔗糖依赖性粘附率降低。对gtfB和gtfC基因序列的测定表明，gtfB和gtfC基因之间的区域约有3500bp的缺失。此外，与MT8148相比，这些菌株的recA基因表达升高。这些结果表明，RecA在gtfB和gtfC基因的融合中起重要作用，导致菌落形态的改变和蔗糖依赖性粘附的减少。
Streptococcus mutans produces 3 types of glucosyltransferases (GTFs), whose cooperative action is essential for cellular adhesion. The recombinase A (RecA) protein is required for homologous recombination. In our previous study, we isolated several strains with a smooth colony morphology and low GTF activity, characteristics speculated to be derived from the GTF fusions. The purpose of the present study was to investigate the mechanism of those fusions. S. mutans strain MT8148 was grown in the presence of recombinant RecA (rRecA) protein, after which smooth colonies were isolated. The biological functions and sequences of the gtfB and gtfC genes of this as well as other clinical strains were determined. The sucrose-dependent adherence rates of those strains were reduced as compared to that of MT8148. Determination of the sequences of the gtfB and gtfC genes showed that an approximately 3500 bp region was deleted from the area between them. Furthermore, expression of the recA gene was elevated in those strains as compared to MT8148. These results suggest that RecA has an important role in fusions of gtfB and gtfC genes, leading to alteration of colony morphology and reduction in sucrose-dependent adhesion.
登录
查看更多内容
影响因子：
--
作者：
Matsumoto, M;Fujita, K;Ooshima, T
通讯作者：
Ooshima, T
影响因子：
15.9
作者：
Cvitkovitch, DG;Liu, YH;Ellen, RP
通讯作者：
Ellen, RP
影响因子：
14.9
作者：
THOMPSON, JD;HIGGINS, DG;GIBSON, TJ
通讯作者：
GIBSON, TJ
影响因子：
7.6
作者：
HAMADA, S;KOGA, T;OOSHIMA, T
通讯作者：
OOSHIMA, T
影响因子：
3.6
作者：
MARTIN, B;GARCIA, P;CLAVERYS, JP
通讯作者：
CLAVERYS, JP