Th9/IL-9 profile in human echinococcosis: their involvement in immune response during infection by Echinococcus granulosus.
Th9/IL-9 profile in human echinococcosis: their involvement in immune response during infection by Echinococcus granulosus.
复制标题
人棘球菌中的TH9/IL-9谱：它们在蠕虫粒颗粒感染过程中参与免疫反应。
DOI：
10.1155/2014/781649
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发表时间：
2014
影响因子：
4.6
通讯作者：
Ding J
中科院分区：
文献类型：
--
作者：
Pang N;Zhang F;Ma X;Zhang Z;Zhao H;Xin Y;Wang S;Zhu Y;Wen H;Ding J
据报道，Th9 细胞有助于免疫反应；然而，Th9细胞在细粒棘球绦虫感染中的作用尚不清楚。本研究旨在确定Th9细胞和IL-9是否参与人类细粒棘球绦虫感染。 qRT-PCR显示，与健康对照（HC组）相比，治疗前患者（CE组）的PU.1、IL-9和GATA-3 mRNA水平显着升高。流式细胞术分析显示CE组Th9和Th2细胞比例明显升高。 CBA检测发现CE组IL-9、IL-4、IL-10、TGF-β水平也显着升高。 CE组Th9和Th2细胞百分比呈正相关。手术联合阿苯达唑治疗后，治疗后患者（PCE组）PU.1和GATA-3 mRNA水平较CE组显着降低。 PCE组Th9和Th2细胞数量以及IL-9、IL-4、IL-10和TGF-β水平也显着降低。总之，治疗后Th9细胞比例和IL-9水平显着降低，提示Th9/IL-9可能参与细粒棘球绦虫感染诱导的免疫反应。
Th9 cells have been reported to contribute to immune responses; however, the role of Th9 cells in Echinococcus granulosus infection is unknown. This study is to determine whether Th9 cells and IL-9 are involved in human Echinococcus granulosus infection. Compared with healthy controls (HC group), the mRNA levels of PU.1, IL-9, and GATA-3 were significantly increased in patients before therapy (CE group), as revealed by qRT-PCR. Flow cytometry analysis showed that the percentages of Th9 and Th2 cells in CE group were significantly higher. The levels of IL-9, IL-4, IL-10, and TGF-β in CE group were also significantly increased, as detected by CBA assay. The percentages of Th9 and Th2 cells in CE group were positively correlated. After treatments of surgery in combination with albendazole, the PU.1 and GATA-3 mRNA levels were significantly decreased in patients after therapy (PCE group) compared with CE group. The numbers of Th9 and Th2 cells and levels of IL-9, IL-4, IL-10, and TGF-β were also significantly decreased in PCE group. In conclusion, the ratios of Th9 cells and IL-9 levels were significantly decreased after treatment, suggesting that Th9/IL-9 may be involved in immune response induced by Echinococcus granulosus infection.
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DOI：
10.4049/jimmunol.1100529
发表时间：
2012-03-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Horka H;Staudt V;Klein M;Taube C;Reuter S;Dehzad N;Andersen JF;Kopecky J;Schild H;Kotsyfakis M;Hoffmann M;Gerlitzki B;Stassen M;Bopp T;Schmitt E
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24.7
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Touil-Boukoffa, Chafia
DOI：
10.3736/jintegrmed2013029
发表时间：
2013-05
期刊：
Journal of integrative medicine
影响因子：
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作者：
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Li XM