Evolution favors protein mutational robustness in sufficiently large populations.
Evolution favors protein mutational robustness in sufficiently large populations.
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DOI：
10.1186/1741-7007-5-29
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发表时间：
2007-07-17
期刊：
影响因子：
5.4
通讯作者：
Arnold FH
中科院分区：
文献类型：
--
作者：
Bloom JD;Lu Z;Chen D;Raval A;Venturelli OS;Arnold FH
一个重要的问题是，进化是否青睐诸如突变稳健性或可进化性之类的特性，这些特性不会直接使任何个体受益，但却能影响未来进化的进程。功能相似的蛋白质在对突变的稳健性以及进化出新功能的能力方面可能存在很大差异，但尚不清楚这些差异是否可能是由于对这些特性的进化选择所致。
在此，我们通过实验室实验证明，如果进化的种群足够大，进化会青睐蛋白质的突变稳健性。我们在不同规模的种群中，在相同的选择压力和突变率下，对细胞色素P450蛋白质进行中性进化，并表明来自更大且因此更多态的种群的蛋白质倾向于具有更高的突变稳健性。来自较大种群的蛋白质还进化出更高的稳定性，这是一种已知能增强突变稳健性和可进化性的生物物理特性。过剩的突变稳健性和稳定性可以通过数学理论很好地描述，并且可以与蛋白质占据其中性网络的方式进行定量关联。
我们的工作首次通过实验证明了在高度多态的种群中进化倾向于青睐突变稳健性和蛋白质稳定性这一普遍趋势。我们认为这种现象可能有助于大量存在的病毒和细菌的突变稳健性和可进化性。
An important question is whether evolution favors properties such as mutational robustness or evolvability that do not directly benefit any individual but can influence the course of future evolution. Functionally similar proteins can differ substantially in their robustness to mutations and capacity to evolve new functions, but it has remained unclear whether any of these differences might be due to evolutionary selection for these properties. Here, we use laboratory experiments to demonstrate that evolution favors protein mutational robustness if the evolving population is sufficiently large. We neutrally evolve cytochrome P450 proteins under identical selection pressures and mutation rates in populations of different sizes, and show that proteins from the larger and thus more polymorphic population tend towards higher mutational robustness. Proteins from the larger population also evolve greater stability, a biophysical property that is known to enhance both mutational robustness and evolvability. The excess mutational robustness and stability is well described by mathematical theory, and can be quantitatively related to the way that the proteins occupy their neutral network. Our work is the first experimental demonstration of the general tendency of evolution to favor mutational robustness and protein stability in highly polymorphic populations. We suggest that this phenomenon could contribute to the mutational robustness and evolvability of viruses and bacteria that exist in large populations.
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DOI：
10.1073/pnas.032668599
发表时间：
2002-02-05
影响因子：
11.1
作者：
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通讯作者：
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DOI：
10.1073/pnas.96.19.10689
发表时间：
1999-09-14
影响因子：
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10.1073/pnas.93.1.397
发表时间：
1996-01-09
影响因子：
11.1
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影响因子：
2.9
作者：
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