TRPM8, a versatile channel in human sperm.
TRPM8, a versatile channel in human sperm.
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DOI：
10.1371/journal.pone.0006095
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发表时间：
2009-06-30
期刊：
影响因子：
3.7
通讯作者：
Treviño CL
中科院分区：
文献类型：
--
作者：
De Blas GA;Darszon A;Ocampo AY;Serrano CJ;Castellano LE;Hernández-González EO;Chirinos M;Larrea F;Beltrán C;Treviño CL
瞬时受体电位通道（TRP）家族包括30多种蛋白质;它们参与各种Ca 2+依赖性过程。TRP在功能上是多样的，涉及调节细胞内Ca 2+（[Ca 2 +]i）浓度的热、化学和机械换能器。Ca 2+在受精过程中触发和/或调节精子的主要功能，如运动性、获能和顶体反应。然而，TRPM亚家族在精子中的存在尚未被探索。在这里，我们的文件与RT-PCR，蛋白质印迹和免疫组化分析TRPM 8在人类精子的存在。我们还研究了使用特定的激动剂（薄荷醇和温度）和拮抗剂（BCTC和辣椒平）的精子功能的参与，这个通道。计算机辅助精子分析显示，薄荷醇没有显着改变人类精子活力。而薄荷醇则诱导人精子顶体反应。辣椒平（20 µM）和BCTC（1.6 µM）分别抑制了约70%和80%的诱导作用。温度或薄荷醇激活TRPM 8可诱导人类精子中[Ca 2 +]i增加，通过群体或单个精子细胞中的荧光测量，辣椒平（20 µM）和BCTC（1.6 µM）也可抑制该效应。然而，孕酮和ZP 3诱导的顶体反应不受辣椒平或BCTC的抑制，这表明TRPM 8激活通过不同的信号通路触发这一过程。这是第一份关于人类精子中存在温度敏感通道（TRPM 8）的报告。该通道可能参与细胞信号传导事件，如趋热性或趋化性。
The transient receptor potential channel (TRP) family includes more than 30 proteins; they participate in various Ca2+ dependent processes. TRPs are functionally diverse involving thermal, chemical and mechanical transducers which modulate the concentration of intracellular Ca2+ ([Ca2+]i). Ca2+ triggers and/or regulates principal sperm functions during fertilization such as motility, capacitation and the acrosome reaction. Nevertheless, the presence of the TRPM subfamily in sperm has not been explored. Here we document with RT-PCR, western blot and immunocitochemistry analysis the presence of TRPM8 in human sperm. We also examined the participation of this channel in sperm function using specific agonists (menthol and temperature) and antagonists (BCTC and capsazepine). Computer-aided sperm analysis revealed that menthol did not significantly alter human sperm motility. In contrast, menthol induced the acrosome reaction in human sperm. This induction was inhibited about 70% by capsazepine (20 µM) and 80% by BCTC (1.6 µM). Activation of TRPM8 either by temperature or menthol induced [Ca2+]i increases in human sperm measured by fluorescence in populations or individual sperm cells, effect that was also inhibited by capsazepine (20 µM) and BCTC (1.6 µM). However, the progesterone and ZP3-induced acrosome reaction was not inhibited by capsazepine or BCTC, suggesting that TRPM8 activation triggers this process by a different signaling pathway. This is the first report dealing with the presence of a thermo sensitive channel (TRPM8) in human sperm. This channel could be involved in cell signaling events such as thermotaxis or chemotaxis.
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影响因子：
1.7
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通讯作者：
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