Manipulation of plant innate immunity and gibberellin as factor of compatibility in the mutualistic association of barley roots with Piriformospora indica.
Manipulation of plant innate immunity and gibberellin as factor of compatibility in the mutualistic association of barley roots with Piriformospora indica.
复制标题
操纵植物先天免疫和赤霉素作为大麦根与印度梨形孢菌互利关联中的相容性因素
DOI：
10.1111/j.1365-313x.2009.03887.x
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Sonnewald
中科院分区：
文献类型：
--
作者：
Schäfer;Pfiffi;Chandler;Waller;Scholz;Pons-Kühnemann;Sonnewald;Sonnewald
担子菌目(Sebacinales)真菌与多种植物存在着广泛的共生共生关系，因而具有独特的生防潜力。作为该菌目的模式生物，印度梨形孢霉能够提高作物的生物量和粮食产量，并诱导局部和系统对真菌疾病的抗性和对非生物胁迫的耐受。为了阐明根部定植的分子基础，我们结合全球基因表达谱、代谢谱和遗传学研究，描述了大麦根与野生稻的相互作用。在代谢水平上，我们发现真菌侵染降低了根尖中游离糖和氨基酸的利用率。在转录水平上，从穿透前相关事件到根部定植的连续相互作用阶段显示了信号感知和转导成分、次生代谢和与膜运输相关的基因的不同调控。此外，我们观察到植物激素代谢相关基因的阶段特异性上调，主要包括赤霉素、生长素和脱落酸，但水杨酸相关基因的表达受到抑制。激素动态平衡的变化伴随着对植物天然免疫系统的普遍抑制。进一步的遗传研究表明，在赤霉素合成和感知能力受损的突变体中，真菌定殖率降低，并暗示赤霉素是根的基础防御的调节剂。我们的数据进一步揭示了宿主-微生物相互作用中亲和性机制的复杂性，并确定赤霉素信号是成功真菌的潜在靶标。
Fungi of the orderSebacinales(Basidiomycota) are involved in a wide spectrum of mutualistic symbioses with various plants, thereby exhibiting unique potential for biocontrol strategies.Piriformospora indica, a model organism of this fungal order, is able to increase the biomass and grain yield of crop plants, and induces local and systemic resistance to fungal diseases and tolerance to abiotic stress. To elucidate the molecular basis for root colonization, we characterized the interaction ofP. indicawith barley roots by combining global gene expression profiling, metabolic profiling, and genetic studies. At the metabolic level, we show that fungal colonization reduces the availability of free sugars and amino acids to the root tip. At the transcriptional level, consecutive interaction stages covering pre‐penetration‐associated events and progressing through to root colonization showed differential regulation of signal perception and transduction components, secondary metabolism, and genes associated with membrane transport. Moreover, we observed stage‐specific up‐regulation of genes involved in phytohormone metabolism, mainly encompassing gibberellin, auxin and abscisic acid, but salicylic acid‐associated gene expression was suppressed. The changes in hormone homoeostasis were accompanied with a general suppression of the plant innate immune system. Further genetic studies showed reduced fungal colonization in mutants that are impaired in gibberellin synthesis as well as perception, and implicate gibberellin as a modulator of the root’s basal defence. Our data further reveal the complexity of compatibility mechanisms in host–microbe interactions, and identify gibberellin signaling as potential target for successful fungi.
登录
查看更多内容
影响因子：
9.5
作者：
Elena Bray Speth;Y. Lee;S. He
通讯作者：
Elena Bray Speth;Y. Lee;S. He
影响因子：
11.4
作者：
Radutoiu, Simona;Madsen, Lene H.;Stougaard, Jens
通讯作者：
Stougaard, Jens
影响因子：
--
作者：
M. Stitt;R. Lilley;Richard Gerhardt;H. Heldt
通讯作者：
M. Stitt;R. Lilley;Richard Gerhardt;H. Heldt
大麦（Hordeum vulgare cv. Salome）叶中茉莉酸甲酯诱导型脂氧合酶的表征。
DOI：
--
发表时间：
1998
期刊：
European Journal of Biochemistry
影响因子：
--
作者：
Kirsten Vörös;Ivo Feussner;Hartmut Kühn;Justin E. E. Lee;Andreas Graner;Marian Löbler;Benno Parthier;Claus Wasternack
通讯作者：
Claus Wasternack
影响因子：
14.9
作者：
Edgar, R;Domrachev, M;Lash, AE
通讯作者：
Lash, AE