Mechanical micronization of lipoaspirates for the treatment of hypertrophic scars
Mechanical micronization of lipoaspirates for the treatment of hypertrophic scars
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脂肪抽吸物机械微粉化治疗肥厚性疤痕
DOI：
10.1186/s13287-019-1140-1
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发表时间：
2019-01
影响因子：
7.5
通讯作者：
Cai Junrong
中科院分区：
文献类型：
--
作者：
Wang Jing;Liao Yunjun;Xia Jing;Wang Zijue;Mo Xiaopei;Feng Jingwei;He Yunfan;Chen Xihang;Li Ye;Lu Feng;Cai Junrong
背景增生性瘢痕给患者带来美容和功能方面的问题，其治疗仍然具有挑战性。脂肪组织的机械微细化可以去除脂肪细胞，浓缩功能细胞。基质血管组分（SVF）-凝胶通过一系列简单的机械过程获得，包括在注射器之间移动和离心。本研究旨在评价SVF凝胶对兔耳增生性瘢痕的治疗作用。从兔腹股沟脂肪垫中获得SVF凝胶和SVF细胞，并注射到疤痕中。使用磷酸盐缓冲盐水（PBS）作为对照。通过组织学和免疫组织化学分析对瘢痕进行结构表征。炎症和纤维化基因的表达进行了evaluated.ResultsHypertrophic瘢痕变得不那么明显，更软的SVF凝胶或SVF细胞注射后。在用SVF-凝胶和SVF细胞处理的组中，真皮厚度显著低于PBS处理组。用SVF-凝胶治疗恢复了疤痕中的皮下脂肪组织，而用SVF细胞和PBS治疗则没有。注射SVF-凝胶和SVF细胞减少真皮层中的巨噬细胞浸润，并降低白细胞介素-6和单核细胞趋化蛋白-1的mRNA表达。此外，肌成纤维细胞和胶原沉积的水平在SVF-gel和SVF cells.ConclusionsSVF-gel治疗组减少增生性瘢痕。将SVF-凝胶注射到增生性瘢痕中可恢复皮下脂肪组织并降低巨噬细胞和肌成纤维细胞的水平;因此，其降低了瘢痕的真皮厚度。
BackgroundHypertrophic scars cause cosmetic and functional problems for patients, and their treatment remains challenging. Mechanical micronization of adipose tissue can remove adipocytes and concentrate functional cells. Stromal vascular fraction (SVF)-gel is obtained by a series of simple mechanical processes, including shifting between syringes and centrifugation. This study aimed to assess the therapeutic effect of SVF-gel on hypertrophic scars.MethodsA model of hypertrophic scars was established in rabbit ears. SVF-gel and SVF cells were obtained from rabbit inguinal fat pads and injected into scars. Phosphate-buffered saline (PBS) was used as a control. Scars were structurally characterized by histologic and immunohistochemical analyses. Expression of inflammatory and fibrogenic genes was evaluated.ResultsHypertrophic scars became less visible and softer following injection of SVF-gel or SVF cells. Dermal thickness was significantly lower in the groups treated with SVF-gel and SVF cells than in the PBS-treated group. Treatment with SVF-gel restored subcutaneous fat tissue in scars, while treatment with SVF cells and PBS did not. Injection of SVF-gel and SVF cells reduced macrophage infiltration in the dermal layer and decreased mRNA expression of interleukin-6 and monocyte chemoattractant protein-1. In addition, the level of myofibroblasts and collagen deposition were reduced in the groups treated with SVF-gel and SVF cells.ConclusionsSVF-gel has therapeutic effects on hypertrophic scars. Injection of SVF-gel into hypertrophic scars restores subcutaneous fat tissue and reduces the levels of macrophages and myofibroblasts; thus, it decreased the dermal thickness of the scar.
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影响因子：
2.9
作者：
J. V. van Dongen;H. Stevens;Mojtaba Parvizi;B. van der Lei;M. Harmsen
通讯作者：
J. V. van Dongen;H. Stevens;Mojtaba Parvizi;B. van der Lei;M. Harmsen
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影响因子：
3.7
作者：
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通讯作者：
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影响因子：
3.6
作者：
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通讯作者：
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影响因子：
4.9
作者：
Bakhtiar H;Mazidi S A;Mohammadi Asl S;Ellini MR;Moshiri A;Nekoofar MH;Dummer PMH
通讯作者：
Dummer PMH
影响因子：
7.5
作者：
Liu YL;Liu WH;Sun J;Hou TJ;Liu YM;Liu HR;Luo YH;Zhao NN;Tang Y;Deng FM
通讯作者：
Deng FM