MTOC formation during mitotic exit in fission yeast.
MTOC formation during mitotic exit in fission yeast.
复制标题
裂殖酵母有丝分裂退出过程中 MTOC 的形成。
作者：
Molly J. Heitz;Janni Petersen;S. Valovin;I. Hagan
微管从含有γ微管蛋白的成核模板聚合。这些模板通常集中在称为微管组织中心（MTOC）的离散结构中。在粟酒裂殖酵母中，赤道MTOC（EMTOC）在后期B的中途形成，然后在细胞分离的最后阶段解体。我们发现，EMTOC是通过招募γ微管蛋白赤道F-肌动蛋白环收缩分裂细胞在两个胞质分裂过程中之前产生的。EMTOC不是一个连续的环。它有一个可变的结构，从马蹄铁到一些短酒吧。EMTOC的完整性依赖于F-肌动蛋白的完整性，但不是微管细胞骨架。EMTOC大会需要的分隔诱导网络（SIN），调节胞质分裂和后期促进复合物的发病活动。SIN在间期细胞中的激活诱导F-肌动蛋白环的形成和收缩以及初级隔膜的合成，但不促进EMTOC组装。与此相反，过度生产的polo样激酶，Plo 1，这也引起了多轮的分隔间期细胞，诱导EMTOC的形成。因此，网络管理EMTOC的形成共享许多控制胞质分裂的调控元件，但更复杂，并揭示了一个额外的功能Plo 1在有丝分裂退出。
Microtubules polymerise from nucleation templates containing gamma tubulin. These templates are generally concentrated in discrete structures called microtubule organising centres (MTOCs). In Schizosaccharomyces pombe, an equatorial MTOC (EMTOC) forms mid-way through anaphase B and then disassembles during the final stages of cell separation. We show that the EMTOC was generated by recruiting gamma tubulin to the equatorial F-actin ring before it constricted to cleave the cell in two during cytokinesis. The EMTOC was not a continuous ring. It had a variable structure ranging from a horseshoe to a number of short bars. EMTOC integrity depended upon the integrity of the F-actin but not the microtubule cytoskeleton. EMTOC assembly required the activity of both the septation-inducing network (SIN) that regulates the onset of cytokinesis and the anaphase-promoting complex. Activation of the SIN in interphase cells induced F-actin ring formation and contraction and the synthesis of the primary septum but did not promote EMTOC assembly. In contrast, overproduction of the polo-like kinase, Plo1, which also induced multiple rounds of septation in interphase cells, induced EMTOC formation. Thus, the network governing EMTOC formation shared many of the regulatory elements that control cytokinesis but was more complex and revealed an additional function for Plo1 during mitotic exit.
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影响因子：
4
作者：
I. Hagan;J. Hyams
通讯作者：
I. Hagan;J. Hyams
影响因子：
16
作者：
Visintin, R;Craig, K;Amon, A
通讯作者：
Amon, A
影响因子：
10.5
作者：
Bridge, AJ;Morphew, M;Hagan, IM
通讯作者：
Hagan, IM
影响因子：
4
作者：
J. Mitchison;P. Nurse
通讯作者：
J. Mitchison;P. Nurse
DOI：
10.1073/pnas.97.11.5919
发表时间：
2000-05-23
影响因子：
11.1
作者：
Flory, MR;Moser, MJ;Davis, TN
通讯作者：
Davis, TN