Synthetic small intestinal scaffolds for improved studies of intestinal differentiation.
Synthetic small intestinal scaffolds for improved studies of intestinal differentiation.
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DOI：
10.1002/bit.25180
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发表时间：
2014-06
影响因子：
3.8
通讯作者：
March, John C.
中科院分区：
文献类型：
--
作者：
Costello, Cait M.;Jia Hongpeng;Shaffiey, Shahab;Yu, Jiajie;Jain, Nina K.;Hackam, David;March, John C.
体外肠道模型可以为小肠功能提供新的见解，包括细胞生长和增殖机制、药物吸收能力和宿主-微生物相互作用。这些模型通常是由在2D支架或transwell插入物上培养的细胞形成的，但人们普遍认为，在3D环境中培养的上皮细胞表现出不同的表型，这些表型更能反映原生组织。我们的重点是开发一种具有绒毛特征的多孔合成3D组织支架，可以支持上皮细胞类型的培养，以模拟小肠的自然微环境。我们证明了我们的支架可以支持Caco-2细胞与产生黏液的细胞系HT29-MTX以及小鼠小肠隐窝的长时间共培养。通过用精确大小的肠绒毛重建表面地形，我们使细胞沿着绒毛轴以类似于天然肠道的方式分化。此外，我们表明，通过在三维模型上培养肠道细胞类型的根尖和底外侧联合饲喂，可以进一步模拟肠道的生化微环境。
In vitro intestinal models can provide new insights into small intestinal function, including cellular growth and proliferation mechanisms, drug absorption capabilities, and host-microbial interactions. These models are typically formed with cells cultured on 2D scaffolds or transwell inserts, but it is widely understood that epithelial cells cultured in 3D environments exhibit different phenotypes that are more reflective of native tissue. Our focus was to develop a porous, synthetic 3D tissue scaffold with villous features that could support the culture of epithelial cell types to mimic the natural microenvironment of the small intestine. We demonstrated that our scaffold could support the co-culture of Caco-2 cells with a mucus-producing cell line, HT29-MTX, as well as small intestinal crypts from mice for extended periods. By recreating the surface topography with accurately sized intestinal villi, we enable cellular differentiation along the villous axis in a similar manner to native intestines. In addition, we show that the biochemical microenvironments of the intestine can be further simulated via a combination of apical and basolateral feeding of intestinal cell types cultured on the 3D models.
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影响因子：
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作者：
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通讯作者：
QUARONI, A
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1152/ajpgi.00090.2006
发表时间：
2006-11-01
影响因子：
4.5
作者：
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