GLH-1/Vasa represses neuropeptide expression and drives spermiogenesis in the C. elegans germline.
GLH-1/Vasa represses neuropeptide expression and drives spermiogenesis in the C. elegans germline.
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DOI：
10.1016/j.ydbio.2022.10.003
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发表时间：
2022-12
影响因子：
2.7
通讯作者：
Updike, Dustin L.
中科院分区：
文献类型：
--
作者：
Rochester, Jesse D.;Min, Hyemin;Gajjar, Gita A.;Sharp, Catherine S.;Maki, Nathaniel J.;Rollins, Jarod A.;Keiper, Brett D.;Graber, Joel H.;Updike, Dustin L.
关键词：
生殖颗粒具有维持生殖系完整性和生殖系干细胞能力的过程。在C. elegans导致生殖细胞的重编程，使它们在第二天的成体中表达体细胞分化的标志物。体细胞重编程与此阶段的完全不育有关。由此产生的生殖细胞萎缩和其他多效性缺陷使我们对重编程的启动以及生殖颗粒内的过程如何保护生殖干细胞的全能性和永生潜力的理解复杂化。为了更好地理解体细胞重编程的初始事件，我们检查了glh-1精确全长缺失中的总mRNA（转录组）和多聚体相关mRNA（翻译组）变化，glh-1编码种系特异性Vasa/DDX 4 DEAD盒RNA解旋酶的同源物。在允许的温度下，生育力的动物作为年轻的成年人进行了分析，该阶段之前的24小时，先前确定的发病的体细胞遗传基因的表达在种系。在这个早期阶段观察到两个重大变化。首先，大多数的神经肽编码转录增加的总和多核糖体mRNA的分数，这表明GLH-1或其效应抑制这种表达。其次，当glh-1缺失时，主要精子蛋白（MSP）结构域mRNA显著减少。我们发现，GLH-1的存在有助于抑制卵子发生过程中的生精表达，但促进MSP的表达，以驱动精子发生和精子运动。这些见解定义了GLH-1在抑制体细胞重编程以维持种系完整性中的早期作用。
Germ granules harbor processes that maintain germline integrity and germline stem cell capacity. Depleting core germ granule components in C. elegans leads to the reprogramming of germ cells, causing them to express markers of somatic differentiation in day-two adults. Somatic reprogramming is associated with complete sterility at this stage. The resulting germ cell atrophy and other pleiotropic defects complicate our understanding of the initiation of reprogramming and how processes within germ granules safeguard the totipotency and immortal potential of germline stem cells. To better understand the initial events of somatic reprogramming, we examined total mRNA (transcriptome) and polysome-associated mRNA (translatome) changes in a precision full-length deletion of glh-1, which encodes a homolog of the germline-specific Vasa/DDX4 DEAD-box RNA helicase. Fertile animals at a permissive temperature were analyzed as young adults, a stage that precedes by 24 h the previously determined onset of somatic reporter-gene expression in the germline. Two significant changes are observed at this early stage. First, the majority of neuropeptide-encoding transcripts increase in both the total and polysomal mRNA fractions, suggesting that GLH-1 or its effectors suppress this expression. Second, there is a significant decrease in Major Sperm Protein (MSP)-domain mRNAs when glh-1 is deleted. We find that the presence of GLH-1 helps repress spermatogenic expression during oogenesis, but boosts MSP expression to drive spermiogenesis and sperm motility. These insights define an early role for GLH-1 in repressing somatic reprogramming to maintain germline integrity.
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影响因子：
7.3
作者：
Ellis RE;Stanfield GM
通讯作者：
Stanfield GM
影响因子：
3.3
作者：
Chen, Wenjun;Hu, Yabing;Lee, Heng-Chi
通讯作者：
Lee, Heng-Chi
影响因子：
3.3
作者：
Ghanta KS;Mello CC
通讯作者：
Mello CC
MIWI/piRNA 在小鼠精子发生过程中的翻译激活功能
影响因子：
64.5
作者：
Dai P;Wang X;Gou LT;Li ZT;Wen Z;Chen ZG;Hua MM;Zhong A;Wang L;Su H;Wan H;Qian K;Liao L;Li J;Tian B;Li D;Fu XD;Shi HJ;Zhou Y;Liu MF
通讯作者：
Liu MF
影响因子：
9.8
作者：
Hoang HD;Miller MA
通讯作者：
Miller MA