A unifying mechanism for protein transport through the core bacterial Sec machinery.
A unifying mechanism for protein transport through the core bacterial Sec machinery.
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作者：
封装和区隔是细胞生命进化的基础，但它们也构成了一个挑战：如何将执行生物功能的分子--蛋白质--越过不可渗透的障碍，进入亚细胞细胞器，并进入细胞外。解决之道在于进化出专门的机器--转体素，这种机器存在于每一种生物膜中，它们既是门又是守门人，跨越和进入膜双层。了解这些转座子如何在分子水平上运作一直是细胞生物学的一个长期抱负，也是一个即将结束的目标；特别是在无处不在的SEC系统的情况下。在这篇综述中，我们强调了最近在结构生物学、生物物理学和生物化学方面改变游戏规则的技术创新的成果，以呈现核心SEC机制的细菌版本的基本完整的机制。我们讨论了我们的模型相对于替代方案的优点，并确定了剩余的未决问题。SEC系统的研究展示的模板对于探索在不同生物膜中发现的许多其他转运蛋白，朝着改变或阻碍其用于制药或生物技术目的功能的最终目标将具有巨大的价值。
Encapsulation and compartmentalization are fundamental to the evolution of cellular life, but they also pose a challenge: how to partition the molecules that perform biological functions—the proteins—across impermeable barriers into sub-cellular organelles, and to the outside. The solution lies in the evolution of specialized machines, translocons, found in every biological membrane, which act both as gate and gatekeeper across and into membrane bilayers. Understanding how these translocons operate at the molecular level has been a long-standing ambition of cell biology, and one that is approaching its denouement; particularly in the case of the ubiquitous Sec system. In this review, we highlight the fruits of recent game-changing technical innovations in structural biology, biophysics and biochemistry to present a largely complete mechanism for the bacterial version of the core Sec machinery. We discuss the merits of our model over alternative proposals and identify the remaining open questions. The template laid out by the study of the Sec system will be of immense value for probing the many other translocons found in diverse biological membranes, towards the ultimate goal of altering or impeding their functions for pharmaceutical or biotechnological purposes.
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影响因子：
5.6
作者：
Zimmer J;Rapoport TA
通讯作者：
Rapoport TA
DOI：
10.1098/rstb.2015.0025
发表时间：
2015-10-05
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Collinson I;Corey RA;Allen WJ
通讯作者：
Allen WJ
影响因子：
7.7
作者：
Alvira S;Watkins DW;Troman L;Allen WJ;Lorriman JS;Degliesposti G;Cohen EJ;Beeby M;Daum B;Gold VA;Skehel JM;Collinson I
通讯作者：
Collinson I
影响因子：
5.6
作者：
Chen, Yu;Bauer, Benedikt W.;Rapoport, Tom A.;Gumbart, James C.
通讯作者：
Gumbart, James C.
影响因子：
3.2
作者：
Bariya, Priya;Randall, Linda L.
通讯作者：
Randall, Linda L.