Effects of equine joint injury on boundary lubrication of articular cartilage by synovial fluid: role of hyaluronan.
Effects of equine joint injury on boundary lubrication of articular cartilage by synovial fluid: role of hyaluronan.
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DOI：
10.1002/art.34520
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发表时间：
2012-09
影响因子：
--
通讯作者：
Sah, Robert L.
中科院分区：
文献类型：
--
作者：
Antonacci, Jennifer M.;Schmidt, Tannin A.;Serventi, Lisa A.;Cai, Matthew Z.;Shu, Yuyu L.;Schumacher, Barbara L.;McIlwraith, C. Wayne;Sah, Robert L.
比较损伤后和对照关节的马滑液（eSF）的软骨边界润滑功能、假定的边界润滑分子透明质酸（HA）、蛋白聚糖-4（PRG 4）和表面活性磷脂（SAPL）、润滑功能和组成之间的关系以及通过添加HA进行的润滑恢复。分析来自正常（NL）、急性损伤（AI）和慢性损伤（CI）关节的eSF，以正常关节软骨的边界润滑作为动摩擦系数（μkinetic）。还分析了eSF的HA、PRG 4和SAPL浓度以及HA分子量（MW）分布。测定了向AI-和NL-eSF样品中加入不同浓度和MW的HA对μ动力学的影响。AI-eSF的μ动力学（0.036）高于NL-eSF（0.026）（+39%）。与NL-eSF相比，AI-eSF具有较低的低分子量形式的HA浓度（-30%），较高的PRG 4浓度（+83%）和较高的SAPL浓度（+144%）。CI-eSF的μ动力学、HA、PRG 4和SAPL特征介于AI-eSF和NL-eSF之间。回归分析显示μ动力学随eSF中HA浓度的增加而降低。单独的HA的减摩性能随着浓度和MW的增加而改善。向AI-eSF中添加高分子量HA（4，000 kDa）将μ动力学降低至接近NL-eSF的值。在急性损伤后阶段，eSF表现出不良的边界润滑特性，如由高μ动力学所指示的。HA的浓度和分子量降低可能是其基础，并将HA添加到缺乏eSF恢复润滑功能。
To compare equine synovial fluid (eSF) from post-injury and control joints for cartilage boundary lubrication function, putative boundary lubricant molecules hyaluronan (HA), proteoglycan-4 (PRG4), and surface-active phospholipids (SAPL), relationships between lubrication function and composition, and lubrication restoration by addition of HA. eSF from normal (NL), acute injury (AI), and chronic injury (CI) joints were analyzed for boundary lubrication of normal articular cartilage as kinetic friction coefficient (μkinetic). eSF were also analyzed for HA, PRG4, and SAPL concentrations and HA molecular weight (MW) distribution. The effect of addition of HA, of different concentrations and MW, to AI- and NL-eSF samples on μkinetic was determined. The μkinetic of AI-eSF (0.036) was higher (+39%) than that of NL-eSF (0.026). Compared to NL-eSF, AI-eSF had a lower HA concentration (−30%) of lower MW forms, higher PRG4 concentration (+83%), and higher SAPL concentration (+144%). CI-eSF had μkinetic, HA, PRG4, and SAPL characteristics intermediate to that of AI-eSF and NL-eSF. Regression analysis revealed that μkinetic decreased with increasing HA concentration in eSF. The friction-reducing properties of HA alone improved with increasing concentration and MW. Addition of high-MW HA (4,000kDa) to AI-eSF reduced μkinetic to a value near that of NL-eSF. In the acute post-injury stage, eSF exhibits poor boundary lubrication properties as indicated by a high μkinetic. HA of diminished concentration and MW may be the basis for this, and adding HA to deficient eSF restored lubrication function.
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影响因子：
5.5
作者：
OGSTON, AG;STANIER, JE
通讯作者：
STANIER, JE
影响因子：
--
作者：
Neu, C. P.;Reddi, A. H.;Komvopoulos, K.;Schmid, T. M.;Di Cesare, P. E.
通讯作者：
Di Cesare, P. E.
影响因子：
2.9
作者：
JAY, GD
通讯作者：
JAY, GD
影响因子：
2.9
作者：
LEE, HG;COWMAN, MK
通讯作者：
COWMAN, MK
影响因子：
--
作者：
BALAZS, EA;WATSON, D;ROSEMAN, S
通讯作者：
ROSEMAN, S