The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination.
The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination.
复制标题
DOI：
10.1186/1471-2164-8-100
复制
发表时间：
2007-04-11
期刊：
影响因子：
4.4
通讯作者：
Jin Q
中科院分区：
文献类型：
--
作者：
Liu T;Zhang Q;Wang L;Yu L;Leng W;Yang J;Chen L;Peng J;Ma L;Dong J;Xu X;Xue Y;Zhu Y;Zhang W;Yang L;Li W;Sun L;Wan Z;Ding G;Yu F;Tu K;Qian Z;Li R;Shen Y;Li Y;Jin Q
分生孢子被认为是红色毛癣菌感染的主要原因。我们建立了一个包含10250个ESTs的cDNA微阵列来监测分生孢子萌发的转录策略。共获得了1561个在该过程中表达水平发生特殊变化的基因，并根据其表达谱进行了分层聚类。通过功能分析，我们提供了一个重要的生物学系统相关的分生孢子萌发，包括在连续的发育阶段的基因表达模式的表征，和相应的形态转变的基因表达谱的变化的全局视图。我们将EST序列与酵母基因组数据库（SGD）中的GO术语进行了匹配。研究发现，酿酒酵母中的一些与信号转导途径和一些重要细胞过程相关的基因同源物参与了T。红花发芽这些基因和信号通路可能在不同的步骤中发挥作用，如激活孢子萌发，维持各向同性生长，建立细胞极性和形态转变。本研究结果有助于在细胞水平上深入了解孢子萌发的分子机制，并有助于加深我们对T.红色。
Conidia are considered to be the primary cause of infections by Trichophyton rubrum. We have developed a cDNA microarray containing 10250 ESTs to monitor the transcriptional strategy of conidial germination. A total of 1561 genes that had their expression levels specially altered in the process were obtained and hierarchically clustered with respect to their expression profiles. By functional analysis, we provided a global view of an important biological system related to conidial germination, including characterization of the pattern of gene expression at sequential developmental phases, and changes of gene expression profiles corresponding to morphological transitions. We matched the EST sequences to GO terms in the Saccharomyces Genome Database (SGD). A number of homologues of Saccharomyces cerevisiae genes related to signalling pathways and some important cellular processes were found to be involved in T. rubrum germination. These genes and signalling pathways may play roles in distinct steps, such as activating conidial germination, maintenance of isotropic growth, establishment of cell polarity and morphological transitions. Our results may provide insights into molecular mechanisms of conidial germination at the cell level, and may enhance our understanding of regulation of gene expression related to the morphological construction of T. rubrum.
登录
查看更多内容
影响因子：
4.8
作者：
Brengues, M;Pintard, L;Lapeyre, B
通讯作者：
Lapeyre, B
影响因子：
11.4
作者：
Herman, PK;Rine, J
通讯作者：
Rine, J
粗糙脉孢菌中的长寡聚体微阵列分析揭示了分生孢子萌发的生化和生理事件背后的转录程序。
影响因子：
14.9
作者：
Kasuga, T;Townsend, JP;Tian, CG;Gilbert, LB;Mannhaupt, G;Taylor, JW;Glass, NL
通讯作者：
Glass, NL
影响因子：
2.8
作者：
Jousson, O;Léchenne, B;Monod, M
通讯作者：
Monod, M
影响因子：
56.9
作者：
Fabrizio, P;Pozza, F;Longo, VD
通讯作者：
Longo, VD