Quantitative Phase Imaging: Recent Advances and Expanding Potential in Biomedicine.
Quantitative Phase Imaging: Recent Advances and Expanding Potential in Biomedicine.
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DOI：
10.1021/acsnano.1c11507
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发表时间：
2022-08-23
期刊：
影响因子：
17.1
通讯作者：
Zangle, Thomas A.
中科院分区：
文献类型：
--
作者：
Nguyen, Thang L.;Pradeep, Soorya;Judson-Torres, Robert L.;Reed, Jason;Teitell, Michael A.;Zangle, Thomas A.
关键词：
定量相位成像（QPI）是一种无标记的宽视场显微镜方法，具有重要的生物医学应用机会。QPI利用光通过透明物体（如哺乳动物细胞）时的自然相移来量化生物量分布以及生物量的时空变化。在60多年前的细胞研究中报道，QPI硬件和软件的不断进步正在导致生物学中的许多应用，在过去的二十年中，其实用性急剧扩展。今天，对细胞大小、形态、行为、细胞粘弹性、药物功效、生物质积累和周转以及运输力学的研究正在支持对发育、生理学、神经活动、癌症以及其他生理过程和疾病的研究。在这里，我们回顾了QPI在生物学领域的基本原理，其次是讨论目前可用或正在开发的技术方法，最后是对正在使用或正在开发的应用程序的广度进行检查。我们评论的优势和缺点，部署QPI在关键的生物医学背景下，并得出结论，新出现的挑战和机遇的基础上结合QPI与其他方法，扩大范围和效用的QPI甚至进一步。
Quantitative phase imaging (QPI) is a label-free, wide-field microscopy approach with significant opportunities for biomedical applications. QPI uses the natural phase shift of light as it passes through a transparent object, such as a mammalian cell, to quantify biomass distribution and spatial and temporal changes in biomass. Reported in cell studies more than 60 years ago, ongoing advances in QPI hardware and software are leading to numerous applications in biology, with a dramatic expansion in utility over the past two decades. Today, investigations of cell size, morphology, behavior, cellular viscoelasticity, drug efficacy, biomass accumulation and turnover, and transport mechanics are supporting studies of development, physiology, neural activity, cancer, and additional physiological processes and diseases. Here, we review the field of QPI in biology starting with underlying principles, followed by a discussion of technical approaches currently available or being developed, and end with an examination of the breadth of applications in use or under development. We comment on strengths and shortcomings for the deployment of QPI in key biomedical contexts and conclude with emerging challenges and opportunities based on combining QPI with other methodologies that expand the scope and utility of QPI even further.
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