Molecular and cellular mechanisms for periodontal diseases: role of Th1 and Th2 type cytokines in induction of mucosal inflammation.
Molecular and cellular mechanisms for periodontal diseases: role of Th1 and Th2 type cytokines in induction of mucosal inflammation.
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牙周疾病的分子和细胞机制：Th1 和 Th2 型细胞因子在诱导粘膜炎症中的作用。
DOI：
10.1111/j.1600-0765.1997.tb01391.x
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发表时间：
1997
影响因子：
3.5
通讯作者：
Kiyono,H
中科院分区：
文献类型：
--
作者：
Yamamoto,M;Fujihashi,K;Hiroi,T;McGhee,JR;VanDyke,TE;Kiyono,H
大量巨噬细胞（巨噬细胞）和产生IG的细胞的积累与成人牙周炎患者的局部和慢性发炎牙龈相关。当从发炎组织中分离牙龈淋巴细胞并通过流式细胞术检查时，大约20-30%的淋巴细胞是CD 4 +T细胞。为了分析这些CD 4 +T细胞的Thl和Th 2细胞因子表达，提取RNA，并通过使用IFN-γ和IL-2（Thl）、IL-4、IL-5、IL-6、IL-10和IL-13（Th 2）以及β-肌动蛋白（管家基因）的特异性5′和3′引物进行逆转录酶聚合酶链反应（RT-PCR）。基于所选择的Thl和Th 2细胞因子的表达，注意到两种不同的细胞因子谱。因此，IFN-γ、IL-6的mRNA表达代表了一种模式。IL-10和IL-13。而另一种情况由IFN-γ、IL-6和IL-13的mRNA组成。除少数病例外，细胞因子特异性RT-PCR未检测到IL-2、IL-4和IL-5的信息。Th 2细胞因子（例如IL-6. IL-10和IL-13）可能有助于诱导局部疾病部位的高B细胞应答。另一方面，IL-4的缺乏可能是导致Mφ在病变牙周组织中积聚的原因。我们还研究了在不存在外源性IL-4的情况下，IL-4受体（IL-4 R）表达与MMP 4持续性之间是否存在关系。与外周血单核细胞（PBMC）中的单核细胞（MN）/巨噬细胞相比，牙龈Mγ表达高水平的IL-4 R mRNA。当牙龈粘膜与重组IL-4（rIL-4）孵育时。细胞存活力因凋亡而显著降低。这些发现清楚地表明，IL-4的缺乏可能导致疾病部位MMPs的持续发生，并且将外源性rIL-4添加到牙龈MMPs培养物中导致细胞凋亡导致细胞死亡。
An accumulation of elevated numbers of macrophages (Mø) and Ig producing cells is associated with localized and chronically inflamed gingiva of patients with adult periodontitis. When gingival lymphocytes were isolated from inflamed tissues and examined by flow cytometry, approximately 20–30% of lymphocytes were CD4+T cells. For the analysis of Thl and Th2 cytokine expression by these CD4+T cells, RNA was extracted and reverse transcriptase polymerase chain reaction (RT‐PCR) was performed by using specific 5′ and 3′primers for IFN‐γ and IL‐2 (Thl), IL‐4, IL‐5, IL‐6, IL‐10 and IL‐13 (Th2) and β‐actin (housekeeping gene). Two distinct cytokine profiles were noted based on the expression of selected Thl and Th2 cytokines. Thus, one pattern was represented by the expression of mRNA for IFN‐γ, IL‐6. IL‐10 and IL‐13. while the other case consisted of mRNA for IFN‐γ, IL‐6 and IL‐13. Except for a few cases, messages for IL‐2, IL‐4 and IL‐5 were not detected by cytokinespecific RT‐PCR. The predominant expression of Th2 cytokines (e.g. IL‐6. IL‐10 and IL‐13) may contribute to the induction of high B cell responses in local disease sites. On the other hand, lack of IL‐4 may be responsible for the accumulation of Mφ in diseased periodontium. We also investigated whether a relationship exists between IL‐4 receptor (IL‐4R) expression and Mø persistence in the absence of exogenous IL‐4. Gingival Mγ, when compared with monocytes (MN)/Mø from peripheral blood mononuclear cells (PBMC), expressed high levels of IL‐4R mRNA. When gingival Mø were incubated with recombinant IL‐4 (rIL‐4). the cell viability was dramatically reduced by apoptosis. These findings clearly show that the lack of IL‐4 may contribute to the persistant occurrence of Mø at the disease site and addition of exogenous rIL‐4 to gingival Mø cultures leads to cell death by apoptosis.
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耶尔森菌引起的关节炎反应性患者滑液中 Th1 型 T 细胞的优势
影响因子：
5.4
作者：
J. Schlaak;E. Hermann;M. Ringhoffer;P. Probst;H. Gallati;K. zum Büschenfelde;B. Fleischer
通讯作者：
B. Fleischer
对慢性发炎牙龈组织的功能性单核细胞进行细胞分析。
DOI：
--
发表时间：
1989
期刊：
Regional immunology
影响因子：
--
作者：
McGhee,ML;Ogawa,T;Pitts,AM;Moldoveanu,Z;Mestecky,J;McGhee,JR;Kiyono,H
通讯作者：
Kiyono,H
DOI：
10.1073/pnas.88.10.4538
发表时间：
1991-05-01
影响因子：
11.1
作者：
PARRONCHI, P;MACCHIA, D;ROMAGNANI, S
通讯作者：
ROMAGNANI, S
类风湿性滑膜炎中白介素 4 水平低，转化生长因子 β 水平高。
影响因子：
--
作者：
Pierre Miossec;Mercedes Naviliat;A. D. d’Angeac;Jacques Sany;J. Banchereau
通讯作者：
J. Banchereau
定义对病原体的保护性反应：麻风病灶中的细胞因子谱。
DOI：
10.1126/science.254.5029.277
发表时间：
1991
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Yamamura,M;Uyemura,K;Deans,RJ;Weinberg,K;Rea,TH;Bloom,BR;Modlin,RL
通讯作者：
Modlin,RL