Vitamin D Deficiency Induces Elevated Oxidative and Biomechanical Damage in Coronary Arterioles in Male Rats.
Vitamin D Deficiency Induces Elevated Oxidative and Biomechanical Damage in Coronary Arterioles in Male Rats.
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DOI：
10.3390/antiox9100997
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发表时间：
2020-10-15
期刊：
影响因子：
--
通讯作者：
Várbíró S
中科院分区：
文献类型：
--
作者：
Sziva RE;Fontányi Z;Pál É;Hadjadj L;Monori-Kiss A;Horváth EM;Benkő R;Magyar A;Heinzlmann A;Benyó Z;Nádasy GL;Várbíró S
背景：一些报告证明维生素D(VD)缺乏与心血管风险增加之间存在内在联系。本研究的目的是探讨血管性痴呆状态对大鼠冠状动脉生物力学和氧化-硝化应激参数的影响。方法：将4周龄雄性Wistar大鼠分为正常对照组(11只)和VD缺乏组(11只)。8周后，取冠状小动脉段。通过体外动脉造影术测量几何、弹性和生物力学特性。用免疫组织化学方法检测O-N应激标志物。结果：VD缺陷组内径减小，壁厚和壁厚/管腔直径比增大，切向壁应力和弹性模量值降低。管壁横截面积、内膜-中膜面积百分比无明显差异。当血管壁弹性成分减少时，α-平滑肌肌动蛋白(α-SMA)免疫染色强度无明显变化。脂质过氧化标记物4-羟基-2-壬烯醛(HNE)显著升高，而其他氧-氮应激标记物(聚(ADP)核糖、3-硝基酪氨酸)染色强度无明显变化。结论：内向富营养性重塑已形成。这些损伤的潜在背景可能涉及氧化损伤标志物(HNE)的初始变化。这些机制可能导致VD虚弱时心血管疾病的发生率增加。
Background: Several reports prove interconnection between vitamin D (VD) deficiency and increased cardiovascular risk. Our aim was to investigate the effects of VD status on biomechanical and oxidative–nitrative (O–N) stress parameters of coronary arterioles in rats. Methods: 4-week-old male Wistar rats were divided into a control group (11 animals) with optimal VD supply (300 IU/kgbw/day) and a VD-deficient group (11 animals, <5 IU/kg/day). After 8 weeks, coronary arteriole segments were prepared. Geometrical, elastic, and biomechanical characteristics were measured by in vitro arteriography. O–N stress markers were investigated by immunohistochemistry. Results: Inner radius decreased; wall thickness and wall-thickness/lumen diameter ratio increased; tangential wall stress and elastic modulus were reduced in VD-deficient group. No difference could be found in wall-cross-sectional area, intima-media area %. While the elastic elements of the vessel wall decreased, the α-smooth muscle actin (α-SMA) immunostaining intensity showed no changes. Significant elevation was found in the lipid peroxidation marker of 4-hidroxy-2-nonenal (HNE), while other O–N stress markers staining intensity (poly(ADP)ribose, 3-nitrotyrosine) did not change. Conclusions: Inward eutrophic remodeling has developed. The potential background of these impairments may involve the initial change in oxidative damage markers (HNE). These mechanisms can contribute to the increased incidence of the cardiovascular diseases in VD deficiency.
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影响因子：
20.3
作者：
Bouillon R;Marcocci C;Carmeliet G;Bikle D;White JH;Dawson-Hughes B;Lips P;Munns CF;Lazaretti-Castro M;Giustina A;Bilezikian J
通讯作者：
Bilezikian J
影响因子：
3
作者：
Argacha, Jean-Francois;Egrise, Dominique;Moreno-Reyes, Rodrigo
通讯作者：
Moreno-Reyes, Rodrigo
影响因子：
4.7
作者：
Bhowmick, Saurav;D'Mello, Veera;Abdul-Muneer, P. M.
通讯作者：
Abdul-Muneer, P. M.
影响因子：
--
作者：
Matrai, Mate;Hetthessy, Judit;Varbiro, Szabolcs
通讯作者：
Varbiro, Szabolcs
影响因子：
5.9
作者：
Kienreich K;Tomaschitz A;Verheyen N;Pieber T;Gaksch M;Grübler MR;Pilz S
通讯作者：
Pilz S