Effect of tissue-harvesting site on yield of stem cells derived from adipose tissue: implications for cell-based therapies.
Effect of tissue-harvesting site on yield of stem cells derived from adipose tissue: implications for cell-based therapies.
复制标题
DOI：
10.1007/s00441-007-0555-7
复制
发表时间：
2008-06
影响因子：
3.6
通讯作者：
van Milligen, Florine J.
中科院分区：
文献类型：
--
作者：
Jurgens, Wouter J. F. M.;Oedayrajsingh-Varma, Maikel J.;Helder, Marco N.;ZandiehDoulabi, Behrouz;Scbouten, Tabitha E.;Kuik, Dirk J.;Ritt, Marco J. P. F.;van Milligen, Florine J.
关键词：
脂肪组织的间质血管部分（SVF）含有大量的多能脂肪组织来源的干细胞（ASC），其具有在体外分化为中胚层谱系细胞的能力。对于基于细胞的疗法，一种有利的方法是在单个外科手术中收获这些SVF细胞并将它们还给患者，从而避免冗长且昂贵的体外培养步骤。然而，这需要SVF分离物含有足够的能够分化成所需细胞谱系的ASC。我们研究了ASC的产量和功能是否受到最常用于收获脂肪组织的解剖部位的影响：腹部和臀部/大腿区域。在有限稀释和集落形成单位（CFU）测定中测定腹部和臀部/大腿区域脂肪组织SVF中ASC的频率。通过定量实时聚合酶链反应和免疫组织化学研究了这些ASCs分化成软骨和成骨途径的能力。从腹部（5.1 ± 1.1%，平均值± SEM）和臀部/大腿区域（1.2 ± 0.7%）采集的脂肪组织之间的ASC频率存在显著差异（P = 0.0009），但有核细胞的绝对数量无显著差异。然而，在来自两种组织的CFU中，具有成骨分化潜能的CFU的频率是相同的。培养时，获得具有相似生长动力学和表型的同质细胞群。从两个组织收获部位的ASC之间的分化能力没有差异。我们的结论是，ASC的产量，但不是每体积的有核细胞的总量或ASC的增殖和分化能力，是依赖于组织收获网站。腹部似乎比臀部/大腿区域更适合用于收获脂肪组织，特别是当考虑在骨骼组织工程的一步外科手术中将SVF细胞用于基于干细胞的治疗时。
The stromal vascular fraction (SVF) of adipose tissue contains an abundant population of multipotent adipose-tissue-derived stem cells (ASCs) that possess the capacity to differentiate into cells of the mesodermal lineage in vitro. For cell-based therapies, an advantageous approach would be to harvest these SVF cells and give them back to the patient within a single surgical procedure, thereby avoiding lengthy and costly in vitro culturing steps. However, this requires SVF-isolates to contain sufficient ASCs capable of differentiating into the desired cell lineage. We have investigated whether the yield and function of ASCs are affected by the anatomical sites most frequently used for harvesting adipose tissue: the abdomen and hip/thigh region. The frequency of ASCs in the SVF of adipose tissue from the abdomen and hip/thigh region was determined in limiting dilution and colony-forming unit (CFU) assays. The capacity of these ASCs to differentiate into the chondrogenic and osteogenic pathways was investigated by quantitative real-time polymerase chain reaction and (immuno)histochemistry. A significant difference (P = 0.0009) was seen in ASC frequency but not in the absolute number of nucleated cells between adipose tissue harvested from the abdomen (5.1 ± 1.1%, mean ± SEM) and hip/thigh region (1.2 ± 0.7%). However, within the CFUs derived from both tissues, the frequency of CFUs having osteogenic differentiation potential was the same. When cultured, homogeneous cell populations were obtained with similar growth kinetics and phenotype. No differences were detected in differentiation capacity between ASCs from both tissue-harvesting sites. We conclude that the yield of ASCs, but not the total amount of nucleated cells per volume or the ASC proliferation and differentiation capacities, are dependent on the tissue-harvesting site. The abdomen seems to be preferable to the hip/thigh region for harvesting adipose tissue, in particular when considering SVF cells for stem-cell-based therapies in one-step surgical procedures for skeletal tissue engineering.
登录
查看更多内容
影响因子：
5.2
作者：
Mitchell, James B.;McIntosh, Kevin;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.
影响因子：
4
作者：
Gronthos, S;Zannettino, ACW;Simmons, PJ
通讯作者：
Simmons, PJ
影响因子：
4.5
作者：
Oedayrajsingh-Varma, MJ;van Ham, SM;van Milligen, FJ
通讯作者：
van Milligen, FJ
影响因子：
2.9
作者：
Asakura, A;Komaki, M;Rudnicki, MA
通讯作者：
Rudnicki, MA
影响因子：
--
作者：
Peterson, B;Zhang, J;Lieberman, JR
通讯作者：
Lieberman, JR