Differential modes of crosslinking establish spatially distinct regions of peptidoglycan in Caulobacter crescentus
Differential modes of crosslinking establish spatially distinct regions of peptidoglycan in Caulobacter crescentus
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不同的交联模式在新月柄杆菌中建立了空间不同的肽聚糖区域
DOI：
10.1111/mmi.14199
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发表时间：
2019
影响因子：
3.6
通讯作者：
Klein, Eric A.
中科院分区：
文献类型：
--
作者：
Stankeviciute, Gabriele;Miguel, Amanda V.;Radkov, Atanas;Chou, Seemay;Huang, Kerwyn Casey;Klein, Eric A.
细菌王国中细胞形状的多样性反映了进化的压力，这些压力产生了生理上重要的形态。虽然已经努力了解一些典型细胞形态的调控，如杆状大肠杆菌，但对大多数细胞形状知之甚少。对于新月柄杆菌，极性柄合成与其二型生活史有关，柄伸长受磷酸盐可用性调节。基于先前的观察，C.由于crescentusstalks具有溶菌酶抗性，我们比较了茎和细胞体的肽聚糖细胞壁的组成，并确定了肽聚糖交联的关键差异。细胞体肽聚糖主要含有内消旋-二氨基庚二酸和D-丙氨酸残基之间的DD-交联，而茎肽聚糖具有更多的LD-转肽作用（内消旋-二氨基庚二酸-内消旋-二氨基庚二酸），由LdtD介导。我们确定ldtD与茎伸长有关;相反，茎LD转肽反映了与茎中低肽聚糖周转相关的老化过程。我们还发现，溶菌酶抗性是LD交联的结构结果。尽管在C. crescentus中，这两种性质之间的相关性在其他生物体中保持，表明DAP-DAP交联可能是调节细菌对溶菌酶敏感性的一般机制。
The diversity of cell shapes across the bacterial kingdom reflects evolutionary pressures that have produced physiologically important morphologies. While efforts have been made to understand the regulation of some prototypical cell morphologies such as that of rod‐shapedEscherichia coli, little is known about most cell shapes. ForCaulobacter crescentus, polar stalk synthesis is tied to its dimorphic life cycle, and stalk elongation is regulated by phosphate availability. Based on the previous observation thatC. crescentusstalks are lysozyme‐resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking. Cell body peptidoglycan contained primarily DD‐crosslinks betweenmeso‐diaminopimelic acid and D‐alanine residues, whereas stalk peptidoglycan had more LD‐transpeptidation (meso‐diaminopimelic acid‐meso‐diaminopimelic acid), mediated by LdtD. We determined thatldtDis dispensable for stalk elongation; rather, stalk LD‐transpeptidation reflects an aging process associated with low peptidoglycan turnover in the stalk. We also found that lysozyme resistance is a structural consequence of LD‐crosslinking. Despite no obvious selection pressure for LD‐crosslinking or lysozyme resistance inC. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP‐DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.
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影响因子：
3.9
作者：
GOODWIN, SD;SHEDLARSKI, JG
通讯作者：
SHEDLARSKI, JG
新月柄杆菌中的胞壁质结构以及 DD 和 LD 羧肽酶活性的缺乏
影响因子：
3.2
作者：
Zdzislaw Markiewicz;B. Glauner;U. Schwarz
通讯作者：
U. Schwarz
DOI：
10.1002/anie.201206749
发表时间：
2012-12-07
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Kuru E;Hughes HV;Brown PJ;Hall E;Tekkam S;Cava F;de Pedro MA;Brun YV;VanNieuwenhze MS
通讯作者：
VanNieuwenhze MS
Cpx 包络应激反应
影响因子：
20.1
作者：
T. Raivio
通讯作者：
T. Raivio
影响因子：
3.2
作者：
POINDEXTER, JS;HAGENZIEKER, JG
通讯作者：
HAGENZIEKER, JG