Expression of T lymphocyte chemoattractants and activation markers in vernal keratoconjunctivitis
Expression of T lymphocyte chemoattractants and activation markers in vernal keratoconjunctivitis
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春季角结膜炎中T淋巴细胞趋化因子及激活标志物的表达
DOI：
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发表时间：
2002
影响因子：
4.1
通讯作者：
Karel Geboes
中科院分区：
文献类型：
--
作者：
A. M. El;S. Struyf;S. Al;Luc Missotten;J. Damme;Karel Geboes
背景/目标：春季角结膜炎（VKC）患者的结膜中存在数量增加的T淋巴细胞，它们的活化在VKC中观察到的慢性过敏性炎症反应的发病机制中具有核心作用。本研究的目的是检测最近描述的三种有效的T淋巴细胞趋化因子PARC的表达，（肺和活化调节的趋化因子）、巨噬细胞衍生的趋化因子（MDC）和I-309、MDC受体CCR 4和T淋巴细胞活化标志物CD 25、CD 26、CD 62 L、CD 71和CD 30，并与VKC患者结膜CD 3 + T淋巴细胞计数进行相关分析。方法：采用一组抗PARC、MDC、I-309、CCR 4、CD 25、CD 26、CD 62 L、CD 71和CD 30的单克隆和多克隆抗体，通过免疫组织化学技术对11例活动性VKC患者和8例对照受试者的结膜活检标本进行了研究。计数阳性染色细胞数。免疫组化双标法检测炎性细胞表达趋化因子的表型。结果：正常结膜固有质上部血管内皮细胞呈弱阳性反应。其他抗体无免疫反应性。VKC标本显示炎性细胞表达PARC、MDC和I-309。PARC+炎性细胞的数量高于MDC+和I-309+炎性细胞的数量，三组的平均值差异显著（分别为17.0（SD 10.1）; 9.5（9.9）和4.3（7.9），p = 0.0117，ANOVA）。PARC+炎性细胞数与CD 3 + T淋巴细胞数相关性最强。仅在3例标本中观察到少量CCR 4+炎性细胞。双重免疫组化显示所有表达趋化因子的炎性细胞均为CD 68+单核/巨噬细胞。CD 25 + T淋巴细胞数高于CD 26+、CD 62 L+、CD 71+和CD 30 + T淋巴细胞数，且5组间均值差异有显著性（分别为46.2（27.9）、30.7（16.0）、20.1（8.6）、7.8（7.7）和6.5（4.0），p <0.001，ANOVA）。CD 25 + T淋巴细胞数与CD 3 + T淋巴细胞数相关性最强。结论：这些结果表明PARC、MDC和I-309在VKC中吸引T淋巴细胞进入结膜中具有潜在作用。VKC中的T淋巴细胞被激活并表达多种活化标志物，这些活化标志物可能有助于VKC的发病。
Background/aims: T lymphocytes are present in increased numbers in the conjunctiva of patients with vernal keratoconjunctivitis (VKC) and their activation has a central role in the pathogenesis of the chronic allergic inflammatory reactions seen in VKC. The aims of this study were to examine the expression of three recently described potent T lymphocyte chemoattractants, PARC (pulmonary and activation regulated chemokine), macrophage derived chemokine (MDC), and I-309, the MDC receptor CCR4, and T lymphocyte activation markers, CD25, CD26, CD62L, CD71, and CD30, and to correlate them with the counts of CD3+ T lymphocytes in the conjunctiva of patients with VKC. Method: Conjunctival biopsy specimens from 11 patients with active VKC, and eight control subjects were studied by immunohistochemical techniques using a panel of monoclonal and polyclonal antibodies directed against PARC, MDC, I-309, CCR4, CD25, CD26, CD62L, CD71, and CD30. The numbers of positively stained cells were counted. The phenotype of inflammatory cells expressing chemokines was examined by double immunohistochemistry. Results: In the normal conjunctiva, vascular endothelial cells in the upper substantia propria showed weak immunoreactivity for CD26. There was no immunoreactivity for the other antibodies. VKC specimens showed inflammatory cells expressing PARC, MDC, and I-309. The numbers of PARC+ inflammatory cells were higher than the numbers of MDC+ and I-309+ inflammatory cells and the mean values of the three groups differed significantly (17.0 (SD 10.1); 9.5 (9.9), and 4.3 (7.9), respectively, p = 0.0117, ANOVA). The numbers of PARC+ inflammatory cells had the strongest correlation with the numbers of CD3+ T lymphocytes. Few CCR4+ inflammatory cells were observed in only three specimens. Double immunohistochemistry revealed that all inflammatory cells expressing chemokines were CD68+ monocytes/macrophages. The numbers of CD25+ T lymphocytes were higher than the numbers of CD26+, CD62L+, CD71+, and CD30+ T lymphocytes and the mean values of the five groups differed significantly (46.2 (27.9), 30.7 (16.0), 20.1 (8.6), 7.8 (7.7), and 6.5 (4.0), respectively, p <0.001, ANOVA). The numbers of CD25+ T lymphocytes had the strongest correlation with the numbers of CD3+ T lymphocytes. Conclusion: These results suggest a potential role for PARC, MDC, and I-309 in attracting T lymphocytes into conjunctiva in VKC. T lymphocytes in VKC are activated and express several activation markers which might contribute to the pathogenesis of VKC.
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影响因子：
21.1
作者：
P. Murphy;M. Baggiolini;I. Charo;C. Hébert;R. Horuk;K. Matsushima;L. Miller;J. Oppenheim;C. Power-C.
通讯作者：
P. Murphy;M. Baggiolini;I. Charo;C. Hébert;R. Horuk;K. Matsushima;L. Miller;J. Oppenheim;C. Power-C.
T 细胞在体外通过内皮细胞单层迁移的近期和长期激活的证据。
DOI：
--
发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Masuyama,J;Berman,JS;Cruikshank,WW;Morimoto,C;Center,DM
通讯作者：
Center,DM
正常淋巴组织和发炎的淋巴外组织中白细胞 L-选择素 (CD62L) 表达的差异调节。
影响因子：
3.4
作者：
Munro,JM;Briscoe,DM;Tedder,TF
通讯作者：
Tedder,TF
影响因子：
14.2
作者：
Bochner, BS;Bickel, CA;Godiska, R
通讯作者：
Godiska, R
影响因子：
4.4
作者：
M. Alzona;Hans-Martin Jäck;R. Fisher;T. M. Ellis
通讯作者：
M. Alzona;Hans-Martin Jäck;R. Fisher;T. M. Ellis