Developmental AMPA receptor subunit specificity during experience-driven synaptic plasticity in the rat barrel cortex.
Developmental AMPA receptor subunit specificity during experience-driven synaptic plasticity in the rat barrel cortex.
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DOI：
10.1016/j.brainres.2011.11.033
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发表时间：
2012-01-30
期刊：
影响因子：
2.9
通讯作者：
Takahashi T
中科院分区：
文献类型：
--
作者：
Miyazaki T;Kunii M;Tada H;Sano A;Kuroiwa Y;Goto T;Malinow R;Takahashi T
在出生后的早期大脑发育过程中，经验驱动的AMPA受体传递到突触参与了皮层功能的初始组织。通过结合病毒介导的体内基因传递与体外全细胞记录，我们确定了一个亚单位特定的发展计划的经验驱动的AMPA受体传递到突触在大鼠桶皮质。我们表达绿色荧光蛋白（GFP）标记的AMPA受体（GFP-GluR 1，或GFP-GluR 4）到2/3层锥体神经元在两个不同的发育时期，出生后（P）8-P10和P12-P14。病毒感染后两天，制备急性脑切片，通过全细胞记录分析从第4层到第2/3层的突触传递。我们发现，胡须的经验驱动GluR 4，而不是GluR 1进入这些突触在出生后发育早期（P8-P10）。然而，在P12-14，GluR 1而不是GluR 4通过触须经验被递送到突触中。这种精确的发育计划表明，不同AMPA受体亚基具有独特的可塑性，这些亚基形成了最初的经验驱动的皮层功能组织。
During early postnatal brain development, experience-driven delivery of AMPA receptors to synapses participates in the initial organization of cortical function. By combining virus-mediated in vivo gene delivery with in vitro whole cell recordings, we identified a subunit-specific developmental program of experience-driven AMPA receptor delivery to synapses in rat barrel cortex. We expressed green fluorescent protein (GFP)-tagged AMPA receptors (GFP-GluR1, or GFP-GluR4) into layer 2/3 pyramidal neurons at two distinct developmental periods, postnatal day (P)8–P10 and P12–P14. Two days after viral infection, acute brain slices were prepared, and synaptic transmission from layer 4 to layer 2/3 was analyzed by whole cell recordings. We found that whisker experience drives GluR4 but not GluR1 into these synapses early in postnatal development (P8–P10). However, at P12–14, GluR1 but not GluR4 is delivered into synapses by whisker experience. This precise developmental plan suggests unique plasticity properties endowed in different AMPA receptor subunits which shape the initial experience-driven organization of cortical function.
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影响因子：
56.9
作者：
Rumpel, S;LeDoux, J;Malinow, R
通讯作者：
Malinow, R
影响因子：
16.2
作者：
Stern, EA;Maravall, M;Svoboda, K
通讯作者：
Svoboda, K
DOI：
10.1073/pnas.0501509102
发表时间：
2005-04-12
影响因子：
11.1
作者：
Esaki, T;Cook, M;Holmes, A
通讯作者：
Holmes, A
影响因子：
2.5
作者：
DURHAM, D;WOOLSEY, TA
通讯作者：
WOOLSEY, TA
影响因子：
16.2
作者：
Kessels, Helmut W.;Malinow, Roberto
通讯作者：
Malinow, Roberto