Transcriptomic analysis reveals new regulatory roles of Clp signaling in secondary metabolite biosynthesis and surface motility in Lysobacter enzymogenes OH11.
Transcriptomic analysis reveals new regulatory roles of Clp signaling in secondary metabolite biosynthesis and surface motility in Lysobacter enzymogenes OH11.
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转录组分析揭示了 Clp 信号在溶杆菌 OH11 次生代谢物生物合成和表面运动中的新调节作用
DOI：
10.1007/s00253-014-6072-1
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发表时间：
2014-11
影响因子：
5
通讯作者：
Liu, Fengquan
中科院分区：
文献类型：
--
作者：
Wang, Yansheng;Zhao, Yuxin;Zhang, Juan;Zhao, Yangyang;Shen, Yan;Su, Zhenhe;Xu, Gaoge;Du, Liangcheng;Huffman, Justin M.;Venturi, Vittorio;Qian, Guoliang;Liu, Fengquan
产酶溶杆菌（Lysobacter enzymogenes）是一种细菌生物控制剂，作为抗生素代谢物的新来源而出现，如HSAF（热稳定抗真菌因子）和抗菌因子WAP-8294 A2。在L.酶基因Clp是一种cAMP受体样蛋白，在L.酶基因然而，Clp信号传导的遗传基础仍不清楚。在此，我们利用转录组/微阵列分析来确定L.酶基因我们发现，Clp是一个全球性的调节基因表达，属于19个功能组的775个基因的转录差异控制Clp信号。分析的Clp调节子检测以前的特点Clp调制的功能，以及新的基因座。这些包括新的基因座参与的植物代谢物的生物合成和表面运动的L。酶基因我们进一步通过实验证明，Clp信号在调节HSAF和WAP-8294 A2的生物合成以及IV型纤毛依赖性性状的表面运动性中起着积极的作用。Clp信号对抗生素（HSAF和WAP-8294 A2）生物合成和表面运动的调节被发现是独立的。重要的是，我们确定了一个因子Lat（溶杆菌乙酰转移酶），组蛋白乙酰转移酶Hpa 2的同源物，这是由Clp调节，并参与HSAF的生物合成，但不与WAP-8294 A2的生产和表面运动。总之，我们的研究为了解Clp信号在L.酶基因
Lysobacter enzymogenes is a bacterial biological-control agent emerging as a new source of antibiotic metabolites, such as HSAF (Heat-Stable Antifungal Factor) and the antibacterial factor WAP-8294A2. The regulatory mechanism(s) for antibiotic-metabolite biosynthesis remains largely unknown in L. enzymogenes. Clp, a cAMP-receptor-like protein, is shown to function as a global regulator in modulating biocontol-associated traits in L. enzymogenes. However, the genetic basis of Clp signaling remains unclear. Here, we utilized transcriptome/microarray analysis to determine the Clp regulon in L. enzymogenes. We showed that Clp is a global regulator in gene expression, as the transcription of 775 genes belonging to 19 functional groups was differentially controlled by Clp signaling. Analysis of the Clp regulon detected previously characterized Clp-modulated functions as well as novel loci. These include novel loci involved in antibiotic-metabolite biosynthesis and surface motility in L. enzymogenes. We further showed experimentally that Clp signaling played a positive role in regulating the biosynthesis of HSAF and WAP-8294A2, as well as surface motility which is a Type-IV-pilus-dependent trait. The regulation by Clp signaling of antibiotic (HSAF and WAP-8294A2) biosynthesis and surface motility was found to be independent. Importantly, we identified a factor Lat (Lysobacter acetyltransferase), a homologue of histone acetyltransferase Hpa2, which was regulated by Clp and involved in HSAF biosynthesis, but not associated with WAP-8294A2 production and surface motility. Overall, our study provided new insights into the regulatory role and molecular mechanism of Clp signaling in L. enzymogenes.
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影响因子：
5.6
作者：
Angus-Hill, ML;Dutnall, RN;Ramakrishnan, V
通讯作者：
Ramakrishnan, V
DOI：
10.1099/00207713-28-3-367
发表时间：
1978-01-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
CHRISTENSEN, P;COOK, FD
通讯作者：
COOK, FD
影响因子：
4.4
作者：
Kobayashi, DY;Reedy, RM;Yuen, GY
通讯作者：
Yuen, GY
影响因子：
3.5
作者：
Burdman S;Bahar O;Parker JK;De La Fuente L
通讯作者：
De La Fuente L
影响因子：
15
作者：
Lou L;Qian G;Xie Y;Hang J;Chen H;Zaleta-Rivera K;Li Y;Shen Y;Dussault PH;Liu F;Du L
通讯作者：
Du L