Differentially expressed circulating LncRNAs and mRNA identified by microarray analysis in obese patients.
Differentially expressed circulating LncRNAs and mRNA identified by microarray analysis in obese patients.
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通过微阵列分析鉴定肥胖患者差异表达的循环 LncRNA 和 mRNA
DOI：
10.1038/srep35421
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发表时间：
2016-10-21
影响因子：
4.6
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Sun J;Ruan Y;Wang M;Chen R;Yu N;Sun L;Liu T;Chen H
循环中的长非编码RNA(LncRNAs)在许多人类疾病中是有价值的生物标志物。然而，lncRNA生物标志物在肥胖症中尚未被识别。我们的目标是表征肥胖和非肥胖受试者循环中的lncRNA的表达。首先，我们评估了3名肥胖者和3名非肥胖者血液中全基因组循环lncRNA的表达谱。我们发现仅在肥胖者中三种LncRNA(lncRNA-p5549、lncRNA-p21015和lncRNA-p19461)的循环水平显著降低。接下来，我们使用RT-PCR检测了33名肥胖者和33名非肥胖者这三个lncRNAs的表达水平，发现了类似的差异。此外，我们还发现这三种lncRNAs的循环水平与体重指数(BMI)、腰围、腰臀比和空腹胰岛素呈负相关。此外，LncRNA-p19461的表达与稳态模型评估-估计的胰岛素抵抗之间也存在显著的负相关。最后，我们测试了8名肥胖受试者在为期12周的饮食诱导减肥计划后循环中这三种LncRNAs的水平。我们发现只有lncRNA-p19461的表达水平显著增加。总而言之，在肥胖中，循环中的lncRNA被解除了调控。体重减轻引起的这一变化支持了这一观察结果，并暗示了潜在的机制相关性。
Circulating long non-coding RNAs (lncRNAs) serve as valuable biomarkers in a number of human diseases. However, lncRNA biomarkers have yet to be identified in obesity. We aim to characterize circulating lncRNA expression in obese and non-obese human subjects. First, we assessed the genome-wide circulating lncRNA expression profiles in blood from 3 obese and 3 non-obese human subjects. We found a significant decrease in circulating levels of three lncRNAs (lncRNA-p5549, lncRNA-p21015 and lncRNA-p19461) in obese human subjects only. Next, using RT-PCR we measured the expression levels of these three lncRNAs in 33 obese and 33 non-obese human subjects and found similar differences. Moreover, we found a negative correlation between circulating levels of these three lncRNAs and body mass index (BMI), waist circumference, waist to hip ratio and fasting insulin. There was also a significant negative correlation between expression of lncRNA-p19461 and homeostasis model assessment-estimated insulin resistance. Finally, we tested the circulating levels of these three lncRNAs in 8 obese human subjects after a 12-week diet-induced weight loss program. We found that only lncRNA-p19461 expression level significantly increased. In summary, circulating lncRNAs are deregulated in obesity. Weight loss–induced changes in this profile support this observation and suggest a potential mechanistic relevance.
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影响因子：
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DOI：
10.1074/jbc.m110.171975
发表时间：
2011-01-28
期刊：
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影响因子：
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作者：
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影响因子：
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