Bone Tissue Collagen Maturity and Mineral Content Increase With Sustained Hyperglycemia in the KK-Ay Murine Model of Type 2 Diabetes.
Bone Tissue Collagen Maturity and Mineral Content Increase With Sustained Hyperglycemia in the KK-Ay Murine Model of Type 2 Diabetes.
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DOI：
10.1002/jbmr.3365
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发表时间：
2018-05
期刊：
影响因子：
--
通讯作者：
Donnelly E
中科院分区：
文献类型：
--
作者：
Hunt HB;Pearl JC;Diaz DR;King KB;Donnelly E
2型糖尿病（T2DM）在一定骨密度（BMD）下增加骨折风险，这表明T2DM改变骨组织特性独立于骨量。在这项研究中，我们利用傅里叶变换红外（FTIR）成像和高效液相色谱（HPLC）技术评估了高血糖对KK-Ay小鼠T2DM模型骨组织组成特性、酶解胶原交联和晚期糖基化终产物（AGEs）的影响。与KK-aa同窝小鼠（n = 8）相比，KK-Ay小鼠（n = 14）股骨近端骨组织胶原成熟度增加，矿物质含量增加，异质性矿物性质减少。通过戊苷浓度评估的AGE积累，以及非酶交联羟基赖氨酸吡啶啉（HP）和赖氨酸吡啶啉（LP）的浓度，与对照组相比，KK-Ay小鼠股骨近端没有差异。在KK-Ay小鼠中观察到的组织水平组成特性的差异与T2DM中骨质老化和重塑减少的观察结果一致。
Type 2 diabetes mellitus (T2DM) increases fracture risk for a given bone mineral density (BMD), which suggests that T2DM changes bone tissue properties independently of bone mass. In this study, we assessed the effects of hyperglycemia on bone tissue compositional properties, enzymatic collagen crosslinks, and advanced glycation end-products (AGEs) in the KK-Ay murine model of T2DM using Fourier transform infrared (FTIR) imaging and high-performance liquid chromatography (HPLC). Compared to KK-aa littermate controls (n = 8), proximal femoral bone tissue of KK-Ay mice (n = 14) exhibited increased collagen maturity, increased mineral content, and less heterogeneous mineral properties. AGE accumulation assessed by the concentration of pentosidine, as well as the concentrations of the nonenzymatic crosslinks hydroxylysylpyridinoline (HP) and lysyl pyridinoline (LP), did not differ in the proximal femurs of KK-Ay mice compared to controls. The observed differences in tissue-level compositional properties in the KK-Ay mice are consistent with bone that is older and echo observations of reduced remodeling in T2DM.
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DOI：
10.1002/jbmr.2607
发表时间：
2016-01
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Farlay D;Armas LA;Gineyts E;Akhter MP;Recker RR;Boivin G
通讯作者：
Boivin G
影响因子：
4
作者：
Gerdhem, P;Isaksson, A;Obrant, KJ
通讯作者：
Obrant, KJ
影响因子：
4.8
作者：
Eyre, David R.;Weis, Mary Ann;Wu, Jiann-Jiu
通讯作者：
Wu, Jiann-Jiu
DOI：
10.1007/s00198-013-2319-4
发表时间：
2013-09
期刊：
Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA
影响因子：
--
作者：
Karim L;Tang SY;Sroga GE;Vashishth D
通讯作者：
Vashishth D
影响因子：
4.2
作者：
Creecy A;Uppuganti S;Merkel AR;O'Neal D;Makowski AJ;Granke M;Voziyan P;Nyman JS
通讯作者：
Nyman JS