Mechanical characterization of vesicles and cells: A review.
Mechanical characterization of vesicles and cells: A review.
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DOI：
10.1002/elps.201900362
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发表时间：
2020-04
期刊：
影响因子：
2.9
通讯作者：
Dutta P
中科院分区：
文献类型：
--
作者：
Morshed A;Karawdeniya BI;Bandara YMNDY;Kim MJ;Dutta P
囊泡在所有活着的有机体中执行许多基本功能。在将施加的力转化为机械和生物反应的过程中，它们像换能器一样对机械应力做出反应。同时，生物化学和生物物理信号都会影响机械载荷作用下的囊泡响应。近年来，脂质体作为一种潜在的药物载体受到了制药行业的广泛关注，它也可以作为一种人工模拟细胞的系统。这些囊泡能够通过更小的毛孔进入，这使它们成为治疗剂有效到达感染部位的理想候选者。设计具有所需机械性能的囊泡，可以根据需要包裹药物并释放，是这一领域的主要挑战。这一要求导致了通过添加胆固醇、鞘磷脂等来改变双层膜的组成。在本文中，我们综述了各种人工/合成囊泡的制造和表征技术。我们特别关注电场驱动的表征技术来确定囊泡及其膜的不同性质，如弯曲刚性、粘度、电容、电导等，这些特性是其含量和迁移率的指示器。人工小泡、天然小泡和细胞之间的异同在整个手稿中都得到了强调，因为大多数这些人工小泡都是用于细胞模拟功能的。
Vesicles perform many essential functions in all living organisms. They respond like a transducer to mechanical stress in converting the applied force into mechanical and biological responses. At the same time, both biochemical and biophysical signals influence the vesicular response in bearing mechanical loads. In recent years, liposomes, artificial lipid vesicles, have gained substantial attention from the pharmaceutical industry as a prospective drug carrier which can also serve as an artificial cell-mimetic system. The ability of these vesicles to enter through pores of even smaller size makes them ideal candidates for therapeutic agents to reach the infected sites effectively. Engineering of vesicles with desired mechanical properties that can encapsulate drugs and release as required is the prime challenge in this field. This requirement has led to the modifications of the composition of the bilayer membrane by adding cholesterol, sphingomyelin, etc. In this paper, we review the manufacturing and characterization techniques of various artificial/synthetic vesicles. We particularly focus on the electric field-driven characterization techniques to determine different properties of vesicle and its membranes such as bending rigidity, viscosity, capacitance, conductance, etc., which are indicators of their content and mobility. Similarities and differences between artificial vesicles, natural vesicles, and cells are highlighted throughout the manuscript since most of these artificial vesicles are intended for cell mimetic functions.
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影响因子：
46.9
作者：
Alvarez-Erviti, Lydia;Seow, Yiqi;Wood, Matthew J. A.
通讯作者：
Wood, Matthew J. A.
影响因子：
9.7
作者：
Darling EM;Di Carlo D
通讯作者：
Di Carlo D
DOI：
10.1073/pnas.211400898
发表时间：
2001-10-23
影响因子：
11.1
作者：
Ando, T;Kodera, N;Toda, A
通讯作者：
Toda, A
影响因子：
3.4
作者：
Aranda, Said;Riske, Karin A.;Dimova, Rumiana
通讯作者：
Dimova, Rumiana
影响因子：
3.7
作者：
Barthès-Biesel, D;Diaz, A;Dhenin, E
通讯作者：
Dhenin, E