Whole-genome discovery of miRNAs and their targets in wheat (Triticum aestivum L.).
Whole-genome discovery of miRNAs and their targets in wheat (Triticum aestivum L.).
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DOI：
10.1186/1471-2229-14-142
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发表时间：
2014-05-22
影响因子：
5.3
通讯作者：
Yao Y
中科院分区：
文献类型：
--
作者：
Sun F;Guo G;Du J;Guo W;Peng H;Ni Z;Sun Q;Yao Y
microRNAs（miRNAs）是一类小分子非编码RNA，在植物生长发育和逆境胁迫应答中起重要作用。小RNA的测序是了解它们在植物中的数量、多样性、表达和可能作用的起点。在这项研究中，我们进行了小麦miRNAs的全基因组调查，从11个组织，共有323个新的miRNAs属于276个家庭的小麦。miRNA保守性分析从9个高度保守的miRNA家族中鉴定出191个小麦特异性miRNA、2个单子叶植物特异性miRNA和30个小麦特异性变体。为了了解小麦miRNAs的可能作用，我们通过degradome测序确定了124个miRNAs家族的524个潜在靶标，并通过5′ RACE验证了其中一个亚组的切割作用。在对miRNAs及其相关靶基因进行全基因组鉴定和特征分析的基础上，我们进一步鉴定了64个miRNAs在发育或萌发的籽粒中优先表达，这些miRNAs可能在籽粒发育中发挥重要作用。我们在全基因组水平上发现了323个小麦新的miRNAs和124个miRNAs家族的524个靶基因，为进一步研究miRNAs在小麦中的功能奠定了基础。
MicroRNAs (miRNAs) are small, non-coding RNAs playing essential roles in plant growth, development, and stress responses. Sequencing of small RNAs is a starting point for understanding their number, diversity, expression and possible roles in plants. In this study, we conducted a genome-wide survey of wheat miRNAs from 11 tissues, characterizing a total of 323 novel miRNAs belonging to 276 families in wheat. A miRNA conservation analysis identified 191 wheat-specific miRNAs, 2 monocot-specific miRNAs, and 30 wheat-specific variants from 9 highly conserved miRNA families. To understand possible roles of wheat miRNAs, we determined 524 potential targets for 124 miRNA families through degradome sequencing, and cleavage of a subset of them was validated via 5′ RACE. Based on the genome-wide identification and characterization of miRNAs and their associated target genes, we further identified 64 miRNAs preferentially expressing in developing or germinating grains, which could play important roles in grain development. We discovered 323 wheat novel miRNAs and 524 target genes for 124 miRNA families in a genome-wide level, and our data will serve as a foundation for future research into the functional roles of miRNAs in wheat.
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从稻粒中表征独特的小RNA群体。
影响因子：
3.7
作者：
Heisel SE;Zhang Y;Allen E;Guo L;Reynolds TL;Yang X;Kovalic D;Roberts JK
通讯作者：
Roberts JK
影响因子：
14.9
作者：
Kozomara A;Griffiths-Jones S
通讯作者：
Griffiths-Jones S
影响因子：
11.6
作者：
GUBLER, F;KALLA, R;JACOBSEN, JV
通讯作者：
JACOBSEN, JV
影响因子：
9.2
作者：
Addo-Quaye, Charles;Eshoo, Tifani W.;Axtell, Michael J.
通讯作者：
Axtell, Michael J.
影响因子：
5.8
作者：
Addo-Quaye, Charles;Miller, Webb;Axtell, Michael J.
通讯作者：
Axtell, Michael J.