Construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping.
Construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping.
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DOI：
10.1111/j.1365-2052.2006.01564.x
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发表时间：
2007-04
期刊：
影响因子：
2.4
通讯作者：
Moore SS
中科院分区：
文献类型：
--
作者：
McKay SD;Schnabel RD;Murdoch BM;Aerts J;Gill CA;Gao C;Li C;Matukumalli LK;Stothard P;Wang Z;Van Tassell CP;Williams JL;Taylor JF;Moore SS
高密度的全基因组图谱对于基因或标记的排序至关重要，并有助于基因组序列的组装。为了增加牛辐射杂交图谱上标记的密度，从而有助于牛基因组序列的组装，使用Illumina® BeadStation在Roslin-Cambridge 3000 rad牛-仓鼠全基因组辐射杂交板（WGRH 3000）上同时分型大量标记。在5个多重反应中，6738个序列标记位点（STS）标记物在WGRH 3000面板DNA上成功分型。这些STS含有作为牛基因组测序计划的结果而开发的SNP。通常，创建高密度辐射杂交（RH）图最耗时和昂贵的部分是用常规方法对RH板上的标记进行基因分型。使用本文中描述的方法，我们已经开发了一个高密度的全基因组RH图与4690个位点和2701个位点的连锁图，与牛全基因组序列组装（Btau_2.0）在一小部分的时间，它会采取与传统的分型和基因分型方法直接比较。
High-density whole-genome maps are essential for ordering genes or markers and aid in the assembly of genome sequence. To increase the density of markers on the bovine radiation hybrid map, and hence contribute to the assembly of the bovine genome sequence, an Illumina® BeadStation was used to simultaneously type large numbers of markers on the Roslin-Cambridge 3000 rad bovine–hamster whole-genome radiation hybrid panel (WGRH3000). In five multiplex reactions, 6738 sequence tagged site (STS) markers were successfully typed on the WGRH3000 panel DNA. These STSs harboured SNPs that were developed as a result of the bovine genome sequencing initiative. Typically, the most time consuming and expensive part of creating high-density radiation hybrid (RH) maps is genotyping the markers on the RH panel with conventional approaches. Using the method described in this article, we have developed a high-density whole-genome RH map with 4690 loci and a linkage map with 2701 loci, with direct comparison to the bovine whole-genome sequence assembly (Btau_2.0) in a fraction of the time it would have taken with conventional typing and genotyping methods.
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