Analysis of saccharification in Brachypodium distachyon stems under mild conditions of hydrolysis.
Analysis of saccharification in Brachypodium distachyon stems under mild conditions of hydrolysis.
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DOI：
10.1186/1754-6834-1-15
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发表时间：
2008-10-22
影响因子：
6.3
通讯作者：
McQueen-Mason SJ
中科院分区：
文献类型：
--
作者：
Gomez LD;Bristow JK;Statham ER;McQueen-Mason SJ
地锦草是一种优良的牧草模式植物。它是一种小的、容易繁殖的温带牧草，具有快速的生活史和小的基因组。它是一种自育植物，可以利用农杆菌和来自幼胚的愈伤组织高效转化。此外，大量的遗传和基因组资源正以绘制种群图、大型表达序列标签集、T-DNA插入线以及在不久的将来获得完整基因组序列的形式提供给该物种。在双子叶植物和禾本科植物差异最大的细胞壁和生物量研究领域，作为模式物种的发展具有特殊的价值。在此，我们探索了温和的预处理和水解条件对青冈茎段糖化特性的影响，为建立不同遗传材料糖化特性的灵敏筛选奠定了基础。短柄草的非纤维素单糖组成与具有重要农业价值的牧草密切相关，与双子叶模式拟南芥有很大不同。稀酸预处理茎段产生大量糖的释放，并对酶解糖的量产生负面影响。单糖和寡糖分析表明，在我们所用的适度的水解条件下，半纤维素部分是酶活性的主要指标。对处理材料的扫描电子显微镜分析显示，茎段中的纤维逐渐暴露。结果表明，在温和的条件下，纤维素和半纤维素都有不同程度的水解，其中以半纤维素的水解为主。我们预计，本研究确定的次优条件将提供一种灵敏的分析方法来检测短柄蕨属植物间糖化作用的差异，从而为鉴定这一特性发生变化的植物提供一个有用的分析工具。
Brachypodium distachyon constitutes an excellent model species for grasses. It is a small, easily propagated, temperate grass with a rapid life cycle and a small genome. It is a self-fertile plant that can be transformed with high efficiency using Agrobacteria and callus derived from immature embryos. In addition, considerable genetic and genomic resources are becoming available for this species in the form of mapping populations, large expressed sequence tag collections, T-DNA insertion lines and, in the near future, the complete genome sequence. The development of Brachypodium as a model species is of particular value in the areas of cell wall and biomass research, where differences between dicots and grasses are greatest. Here we explore the effect of mild conditions of pretreatment and hydrolysis in Brachypodium stem segments as a contribution for the establishment of sensitive screening of the saccharification properties in different genetic materials. The non-cellulosic monosaccharide composition of Brachypodium is closely related to grasses of agricultural importance and significantly different from the dicot model Arabidopsis thaliana. Diluted acid pretreatment of stem segments produced significant release of sugars and negatively affected the amount of sugars obtained by enzymatic hydrolysis. Monosaccharide and oligosaccharide analysis showed that the hemicellulose fraction is the main target of the enzymatic activity under the modest hydrolytic conditions used in our assays. Scanning electron microscopy analysis of the treated materials showed progressive exposure of fibrils in the stem segments. Results presented here indicate that under mild conditions cellulose and hemicellulose are hydrolysed to differing extents, with hemicellulose hydrolysis predominating. We anticipate that the sub-optimal conditions for hydrolysis identified here will provide a sensitive assay to detect variations in saccharification among Brachypodium plants, providing a useful analytical tool for identifying plants with alterations in this trait.
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影响因子：
11.4
作者：
Lloyd, TA;Wyman, CE
通讯作者：
Wyman, CE
影响因子：
2.9
作者：
Anthon, GE;Barrett, DM
通讯作者：
Barrett, DM
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作者：
Kim, Tae Hyun;Taylor, Frank;Hicks, Kevin B.
通讯作者：
Hicks, Kevin B.
影响因子：
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作者：
Kumar, Rajeev;Wyman, Charles E.
通讯作者：
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作者：
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