Evidence for Weakened Intercellular Coupling in the Mammalian Circadian Clock under Long Photoperiod.
Evidence for Weakened Intercellular Coupling in the Mammalian Circadian Clock under Long Photoperiod.
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DOI：
10.1371/journal.pone.0168954
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Michel S
中科院分区：
文献类型：
--
作者：
Buijink MR;Almog A;Wit CB;Roethler O;Olde Engberink AH;Meijer JH;Garlaschelli D;Rohling JH;Michel S
对于生活在温带地区的动物来说，昼长的季节性变化是它们适应变化的环境条件的生理和行为的重要线索。视交叉上核(SCN)在哺乳动物中被称为中央生物钟，但也可能在适应不同的光周期中发挥重要作用。SCN接收来自视网膜的直接光输入，并能够通过将电活动节奏的波形近似为白天的持续时间来编码白天的长度。改变整个波形需要对SCN内的神经元网络进行重组，改变神经元之间的同步度；然而，潜在的机制尚不清楚。在本研究中，我们使用培养的SCN切片中的PER2：：Luc生物发光成像来表征单细胞水平上的网络动力学，我们的目的是为光周期诱导的相分散中耦合强度的调制作用提供证据。与短光周期相比，长光周期(LP)可引起SCN前部单细胞PER2：：LUC节律峰值时间分布的增加。有趣的是，单细胞周期PER2：：LUC节律的周期变异性在LP的SCN前部也较高，且与峰值时间弥散呈正相关。在PER2：：LUC节律的时间序列数据上应用一种新的、公正的群落检测方法，揭示了两个具有特定空间分布的细胞簇，我们将其定义为背外侧和腹内侧核。节律特征的后HOC分析显示，与前侧SCN的腹内侧束相比，背外侧的单细胞周期变异性更大。我们的结论是，SCN网络中耦合强度的变化是对观察到的单细胞周期变异性变化的合理解释，这可能有助于光周期诱导的相位分布。
For animals living in temperate latitudes, seasonal changes in day length are an important cue for adaptations of their physiology and behavior to the altered environmental conditions. The suprachiasmatic nucleus (SCN) is known as the central circadian clock in mammals, but may also play an important role in adaptations to different photoperiods. The SCN receives direct light input from the retina and is able to encode day-length by approximating the waveform of the electrical activity rhythm to the duration of daylight. Changing the overall waveform requires a reorganization of the neuronal network within the SCN with a change in the degree of synchrony between the neurons; however, the underlying mechanisms are yet unknown. In the present study we used PER2::LUC bioluminescence imaging in cultured SCN slices to characterize network dynamics on the single-cell level and we aimed to provide evidence for a role of modulations in coupling strength in the photoperiodic-induced phase dispersal. Exposure to long photoperiod (LP) induced a larger distribution of peak times of the single-cell PER2::LUC rhythms in the anterior SCN, compared to short photoperiod. Interestingly, the cycle-to-cycle variability in single-cell period of PER2::LUC rhythms is also higher in the anterior SCN in LP, and is positively correlated with peak time dispersal. Applying a new, impartial community detection method on the time series data of the PER2::LUC rhythm revealed two clusters of cells with a specific spatial distribution, which we define as dorsolateral and ventromedial SCN. Post hoc analysis of rhythm characteristics of these clusters showed larger cycle-to-cycle single-cell period variability in the dorsolateral compared to the ventromedial cluster in the anterior SCN. We conclude that a change in coupling strength within the SCN network is a plausible explanation to the observed changes in single-cell period variability, which can contribute to the photoperiod-induced phase distribution.
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影响因子：
3.3
作者：
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通讯作者：
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作者：
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DOI：
10.1073/pnas.1319820111
发表时间：
2014-07-01
影响因子：
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作者：
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