A sodium channel mutation identified in Aedes aegypti selectively reduces cockroach sodium channel sensitivity to type I, but not type II pyrethroids.
A sodium channel mutation identified in Aedes aegypti selectively reduces cockroach sodium channel sensitivity to type I, but not type II pyrethroids.
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DOI：
10.1016/j.ibmb.2010.09.005
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发表时间：
2011-01
影响因子：
3.8
通讯作者：
Dong, Ke
中科院分区：
文献类型：
--
作者：
Hu, Zhaonong;Du, Yuzhe;Nomura, Yoshiko;Dong, Ke
关键词：
电压门控钠通道是拟除虫菊酯类杀虫剂的主要靶标。已在拟除虫菊酯抗性昆虫中发现了大量钠通道基因的点突变，其中许多已被证实降低或取消了非洲爪哇卵母细胞表达的通道对拟除虫菊酯的敏感性。最近，在对拟除虫菊酯产生抗性的伊蚊种群中发现了几个新的钠通道基因突变。其中一个突变是伊蚊钠通道结构域III(IIIS6)第6段的苯丙氨酸(F)到半胱氨酸(C)的变化。奇怪的是，先前的一项研究表明，这种F的丙氨酸取代不会改变溴氰菊酯(一种第二类拟除虫菊酯)对蟑螂钠通道的作用。在这项研究中，我们将拟除虫菊酯敏感的蟑螂钠通道中的F改为C，并检测了非洲爪哇卵母细胞对氯菊酯以及其他五种I型或II型拟除虫菊酯的突变通道敏感性。有趣的是，F到C突变显著降低了三种I型拟除虫菊酯(氯菊酯、NRDC157(一种不含α-氰基的溴氰菊酯类似物)和生物拟除虫菊酯)和生物拟除虫菊酯的通道敏感性，但对三种II型拟除虫菊酯氯氰菊酯、溴氰菊酯和氯氰菊酯没有影响。这些结果证实了F到C突变与氯菊酯抗性有关，并提出了在存在这种特殊突变的害虫种群的控制中考虑I型和II型拟除虫菊酯轮换的可能性。
Voltage-gated sodium channels are the primary target of pyrethroid insecticides. Numerous point mutations in sodium channel genes have been identified in pyrethroid-resistant insect species, and many have been confirmed to reduce or abolish sensitivity of channels expressed in Xenopus oocytes to pyrethroids. Recently, several novel mutations were reported in sodium channel genes of pyrethroid-resistant Aedes mosquito populations. One of the mutations is a phenylalanine (F) to cysteine (C) change in segment 6 of domain III (IIIS6) of the Aedes mosquito sodium channel. Curiously, a previous study showed that alanine substitution of this F did not alter the action of deltamethrin, a type II pyrethroid, on a cockroach sodium channel. In this study, we changed this F to C in a pyrethroid-sensitive cockroach sodium channel and examined mutant channel sensitivity to permethrin as well as five other type I or type II pyrethroids in Xenopus oocytes. Interestingly, the F to C mutation drastically reduced channel sensitivity to three type I pyrethroids, permethrin, NRDC 157 (a deltamethrin analogue lacking the α-cyano group) and bioresemthrin, but not to three type II pyrethroids, cypermethrin, deltamethrin and cyhalothrin. These results confirm the involvement of the F to C mutation in permethrin resistance, and raise the possibility that rotation of type I and type II pyrethroids might be considered in the control of insect pest populations where this particular mutation is present.
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影响因子：
4.1
作者：
O'Reilly, Andrias O.;Khambay, Bhupinder P. S.;Davies, T. G. Emyr
通讯作者：
Davies, T. G. Emyr
影响因子：
1.9
作者：
Brengues, C;Hawkes, NJ;Hemingway, J
通讯作者：
Hemingway, J
影响因子：
4.1
作者：
Du, Yuzhe;Lee, Jung-Eun;Dong, Ke
通讯作者：
Dong, Ke
影响因子：
3.8
作者：
Kawada H;Higa Y;Komagata O;Kasai S;Tomita T;Thi Yen N;Loan LL;Sánchez RA;Takagi M
通讯作者：
Takagi M
影响因子：
4.7
作者：
GAMMON, DW;BROWN, MA;CASIDA, JE
通讯作者：
CASIDA, JE