Widespread distribution of a newly found point mutation in voltage-gated sodium channel in pyrethroid-resistant Aedes aegypti populations in Vietnam.
Widespread distribution of a newly found point mutation in voltage-gated sodium channel in pyrethroid-resistant Aedes aegypti populations in Vietnam.
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DOI：
10.1371/journal.pntd.0000527
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发表时间：
2009-10-06
影响因子：
3.8
通讯作者：
Takagi M
中科院分区：
文献类型：
--
作者：
Kawada H;Higa Y;Komagata O;Kasai S;Tomita T;Thi Yen N;Loan LL;Sánchez RA;Takagi M
埃及伊蚊对光稳定性拟除虫菊酯类杀虫剂的抗性是病媒控制方案的一个主要问题。拟除虫菊酯的目标是昆虫神经元上的电压门控钠通道。与拟除虫菊酯抗性相关的该通道中的单个氨基酸取代是引起昆虫敲低抗性的主要因素之一。虽然kdr已被观察到在几个蚊子物种中，在帕拉基因的点突变尚未得到充分的特点，在Ae。埃及人在越南本研究的目的是确定的类型和频率的突变的帕拉基因在Ae。从越南的旧轮胎中收集的埃及伊蚊。由于氨基酸L1014 F的替换，几个点突变导致昆虫神经系统中电压门控钠通道的不敏感性，这是几种蚊子中最常见的点突变; I1011 M（或V）和V1016 G（或I），据报道与Ae的敲低抗性相关。埃及伊蚊位于第6节，结构域II;和最近发现的氨基酸替换F1269在埃及伊蚊。埃及，位于段6，域III。在70个地点的756只幼虫中，未发现I1011 M或I1011 V或L1014 F突变，仅检测到2只V1016 G杂合子蚊子。然而，结构域III上的F1269 C突变分布广泛，在757个幼虫中的269个个体中具有较高的频率（调查的70个地点中的53个收集点）。F1269 C在越南中部和北部的频率较低，但在邻近大城市的地区和越南南部，除了位于海拔500-1000米的南部山区外，频率较高。在目前的情况下，纯合子F1269 C的总体百分比似乎仍然很低（7.4%）。然而，广泛和不受控制的频繁使用光稳定性拟除虫菊酯可能是一个强大的选择压力，这种突变造成严重的问题，在控制登革热在越南。拟除虫菊酯是一组合成化学品的总称，其结构与从菊花中提取的天然除虫菊酯有关。在越南，光稳定性拟除虫菊酯已被广泛用作疟疾和登革热病媒控制的杀虫剂。最近，Kawada等人发现，从旧轮胎中收集的埃及伊蚊在越南北部对拟除虫菊酯敏感，但在越南南部和中部高地具有抗药性。通过分析突变的存在下，在帕拉位点，这会导致不敏感的电压门控钠离子通道在昆虫神经系统中，最近发现的氨基酸替换区域的第6节的结构域III（F1269 C）被发现广泛分布在越南南部的高频率。我们认为，这个点突变在越南的拟除虫菊酯抗性中起着重要作用。广泛和不受控制地频繁使用光稳定性拟除虫菊酯可能是这种突变增加纯合种群的强大选择压力，这在越南控制登革热方面造成严重问题。
Resistance of Aedes aegypti to photostable pyrethroid insecticides is a major problem for disease-vector control programs. Pyrethroids target the voltage-gated sodium channel on the insects' neurons. Single amino acid substitutions in this channel associated with pyrethroid resistance are one of the main factors that cause knockdown resistance in insects. Although kdr has been observed in several mosquito species, point mutations in the para gene have not been fully characterized in Ae. aegypti populations in Vietnam. The aim of this study was to determine the types and frequencies of mutations in the para gene in Ae. aegypti collected from used tires in Vietnam. Several point mutations were examined that cause insensitivity of the voltage-gated sodium channel in the insect nervous system due to the replacement of the amino acids L1014F, the most commonly found point mutation in several mosquitoes; I1011M (or V) and V1016G (or I), which have been reported to be associated to knockdown resistance in Ae. aegypti located in segment 6, domain II; and a recently found amino acid replacement in F1269 in Ae. aegypti, located in segment 6, domain III. Among 756 larvae from 70 locations, no I1011M or I1011V nor L1014F mutations were found, and only two heterozygous V1016G mosquitoes were detected. However, F1269C mutations on domain III were distributed widely and with high frequency in 269 individuals among 757 larvae (53 collection sites among 70 locations surveyed). F1269C frequencies were low in the middle to north part of Vietnam but were high in the areas neighboring big cities and in the south of Vietnam, with the exception of the southern mountainous areas located at an elevation of 500–1000 m. The overall percentage of homozygous F1269C seems to remain low (7.4%) in the present situation. However, extensive and uncontrolled frequent use of photostable pyrethroids might be a strong selection pressure for this mutation to cause serious problems in the control of dengue fever in Vietnam. Pyrethroid is the general term for a group of synthetic chemicals that are structurally related to natural pyrethrins derived from Chrysanthemum flowers. In Vietnam, photostable pyrethroids have been extensively used as insecticides for malaria and dengue vector control. Recently, Kawada et al. found that Aedes aegypti collected from used tires were susceptible to pyrethroids in the North but were resistant in the South and Central Highlands of Vietnam. By analyzing the presence of mutations in the para locus, which cause insensitivity to voltage-gated sodium channel in the insect nervous system, a recently found amino acid replacement in the area of segment 6 of domain III (F1269C) was found to be widely distributed with high frequency in southern Vietnam. We suggest that this point mutation plays an important role in pyrethroid resistance in Vietnam. Extensive and uncontrolled frequent use of photostable pyrethroids might be a strong selection pressure for this mutation to increase homozygous populations, which cause serious problems in controlling dengue fever in Vietnam.
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影响因子：
4.1
作者：
O'Reilly, Andrias O.;Khambay, Bhupinder P. S.;Davies, T. G. Emyr
通讯作者：
Davies, T. G. Emyr
DOI：
10.1590/s0074-02762003000500020
发表时间：
2003-07-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Macoris, Maria de Lourdes G;Andrighetti, Maria Teresa M;Bracco, José Eduardo
通讯作者：
Bracco, José Eduardo
影响因子：
2.7
作者：
Rajatileka, Shavanthi;Black, William C.;Ranson, Hilary
通讯作者：
Ranson, Hilary
影响因子：
1.9
作者：
Brengues, C;Hawkes, NJ;Hemingway, J
通讯作者：
Hemingway, J
中国按蚊群（双翅目：蚊科）：核糖体 DNA 内转录间隔区 2 序列推断的物种区分和系统发育关系
影响因子：
2.1
作者：
Afrane, YA;Lawson, BW;Yan, GY
通讯作者：
Yan, GY