Occurrence and distribution of antibiotic-resistant bacteria and transfer of resistance genes in Lake Taihu.
Occurrence and distribution of antibiotic-resistant bacteria and transfer of resistance genes in Lake Taihu.
复制标题
太湖抗生素耐药细菌的发生、分布及耐药基因转移.
DOI：
10.1264/jsme2.me13098
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发表时间：
2013
影响因子：
2.2
通讯作者：
Xiao L
中科院分区：
文献类型：
--
作者：
Yin Q;Yue D;Peng Y;Liu Y;Xiao L
抗生素的过度使用加速了自然环境中的抗生素耐药性，特别是淡水，对世界各地的公共卫生产生了潜在的风险。在这项研究中，抗生素耐药性在太湖进行了调查，这是第一次通过培养依赖的方法获得全面的数据。链霉素和氨苄青霉素的耐药率较高，其次是四环素和氯霉素。特别是在西部和北方地区，氨苄青霉素耐药性水平很高。细菌学鉴定结果表明，78株分离株中有62.0%的菌株表现出多重耐药，且存在一定的条件致病菌。ESBLs基因的检出顺序为blaTEM> blaSHV> blaCTMX，其中38.5%的菌株携带Ⅰ类整合酶基因。在所有测试的菌株中，80.8%能够通过接合转移抗生素耐药性。我们还发现了一些新的人源ESBLs和AmpC基因家族。细菌分离株（特别是条件致病菌）中抗生素耐药性的流行和可转移抗生素耐药性的传播令人震惊，清楚地表明迫切需要认识到抗生素耐药性对依赖太湖水消费的人类和动物群体的健康风险。
The overuse of antibiotics has accelerated antibiotic resistance in the natural environment, especially fresh water, generating a potential risk for public health around the world. In this study, antibiotic resistance in Lake Taihu was investigated and this was the first thorough data obtained through culture-dependent methods. High percentages of resistance to streptomycin and ampicillin among bacterial isolates were detected, followed by tetracycline and chloramphenicol. Especially high levels of ampicillin resistance in the western and northern regions were illustrated. Bacterial identification of the isolates selected for further study indicated the prevalence of some opportunistic pathogens and 62.0% of the 78 isolates exhibited multiple antibiotic resistance. The presence of ESBLs genes was in the following sequence: blaTEM > blaSHV > blaCTMX and 38.5% of the isolates had a class I integrase gene. Of all tested strains, 80.8% were able to transfer antibiotic resistance through conjugation. We also concluded that some new families of human-associated ESBLs and AmpC genes can be found in natural environmental isolates. The prevalence of antibiotic resistance and the dissemination of transferable antibiotic resistance in bacterial isolates (especially in opportunistic pathogens) was alarming and clearly indicated the urgency of realizing the health risks of antibiotic resistance to human and animal populations who are dependent on Lake Taihu for water consumption.
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影响因子：
3
作者：
Alpay-Karaoglu, Sengul;Ozgumus, Osman Birol;Yesilgil, Pinar
通讯作者：
Yesilgil, Pinar
影响因子：
12.8
作者：
Garcia-Armisen, Tamara;Vercammen, Ken;Cornelis, Pierre
通讯作者：
Cornelis, Pierre
DOI：
10.1126/science.1220761
发表时间：
2012-08-31
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Forsberg KJ;Reyes A;Wang B;Selleck EM;Sommer MO;Dantas G
通讯作者：
Dantas G
影响因子：
9.4
作者：
Coenye, T;Spilker, T;LiPuma, JJ
通讯作者：
LiPuma, JJ
DOI：
10.1006/bmme.1996.0003
发表时间：
1996-02-01
期刊：
BIOCHEMICAL AND MOLECULAR MEDICINE
影响因子：
--
作者：
Afghani, B;Stutman, HR
通讯作者：
Stutman, HR