Wnt and TGF-beta expression in the sponge Amphimedon queenslandica and the origin of metazoan embryonic patterning.
Wnt and TGF-beta expression in the sponge Amphimedon queenslandica and the origin of metazoan embryonic patterning.
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DOI：
10.1371/journal.pone.0001031
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发表时间：
2007-10-10
期刊：
影响因子：
3.7
通讯作者：
Degnan BM
中科院分区：
文献类型：
--
作者：
Adamska M;Degnan SM;Green KM;Adamski M;Craigie A;Larroux C;Degnan BM
后生动物发育和分化的起源取决于细胞粘附、通讯和协作机制的进化。虽然许多主要细胞信号通路的组成部分已在一系列海绵（多孔动物门）中被确定，但它们在发育中的作用尚未被研究，并且在很大程度上仍然未知。在这里，我们通过研究海绵胚胎发育过程中编码Wnt和TGF-β信号配体的基因表达，迈出了重建海绵和真后生动物最后共同祖先中使用的发育信号系统的第一步。利用最近的海绵Amphimedon queenslandica（Demospongiae）基因组计划中产生的资源，我们已经恢复了编码Wnt和TGF-β信号配体的基因，这些配体在后生动物胚胎的模式化中至关重要。这两个基因从双齿蛇胚胎发育的最早阶段就以高度动态的模式表达。当双齿蛇胚胎开始显示前后极性时，Wnt表达变得定位于后极，并且这种表达持续到游泳幼虫阶段。相反，TGF-β表达在前极最高。与复杂动物一样，海绵Wnt和TGF-β表达模式在发育后期在形成简单组织样结构（色素环）的细胞亚群的模式化过程中相交。在整个发育过程中，Wnt和TGF-β沿前后轴沿着呈放射状表达。我们从Wnt和TGF-β在两栖类中的表达推断，产生海绵、刺胞动物和两侧动物的祖先已经进化出指导形成相对复杂的身体计划的能力，包括轴和组织。Wnt和TGF-β沿着海绵胚胎和幼虫前后轴的径向对称表达模式表明，这些信号通路有助于在最初的后生动物中建立轴向极性。
The origin of metazoan development and differentiation was contingent upon the evolution of cell adhesion, communication and cooperation mechanisms. While components of many of the major cell signalling pathways have been identified in a range of sponges (phylum Porifera), their roles in development have not been investigated and remain largely unknown. Here, we take the first steps toward reconstructing the developmental signalling systems used in the last common ancestor to living sponges and eumetazoans by studying the expression of genes encoding Wnt and TGF-β signalling ligands during the embryonic development of a sponge. Using resources generated in the recent sponge Amphimedon queenslandica (Demospongiae) genome project, we have recovered genes encoding Wnt and TGF-β signalling ligands that are critical in patterning metazoan embryos. Both genes are expressed from the earliest stages of Amphimedon embryonic development in highly dynamic patterns. At the time when the Amphimedon embryos begin to display anterior-posterior polarity, Wnt expression becomes localised to the posterior pole and this expression continues until the swimming larva stage. In contrast, TGF-β expression is highest at the anterior pole. As in complex animals, sponge Wnt and TGF-β expression patterns intersect later in development during the patterning of a sub-community of cells that form a simple tissue-like structure, the pigment ring. Throughout development, Wnt and TGF-β are expressed radially along the anterior-posterior axis. We infer from the expression of Wnt and TGF-β in Amphimedon that the ancestor that gave rise to sponges, cnidarians and bilaterians had already evolved the capacity to direct the formation of relatively sophisticated body plans, with axes and tissues. The radially symmetrical expression patterns of Wnt and TGF-β along the anterior-posterior axis of sponge embryos and larvae suggest that these signalling pathways contributed to establishing axial polarity in the very first metazoans.
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影响因子：
56.9
作者：
Jackson, Daniel J.;Macis, Luciana;Woerheide, Gert
通讯作者：
Woerheide, Gert
影响因子：
3.9
作者：
Adell, Teresa;Thakur, Archana N.;Mueller, Werner E. G.
通讯作者：
Mueller, Werner E. G.
DOI：
10.1073/pnas.0405867102
发表时间：
2005-02-01
影响因子：
11.1
作者：
Botting, JP;Butterfield, NJ
通讯作者：
Butterfield, NJ
影响因子：
2.6
作者：
Degnan, BM;Leys, SP;Larroux, C
通讯作者：
Larroux, C
影响因子：
2.5
作者：
Flowers, VL;Courteau, GR;Venuti, JM
通讯作者：
Venuti, JM