3D graphene oxide-encapsulated gold nanoparticles to detect neural stem cell differentiation.
3D graphene oxide-encapsulated gold nanoparticles to detect neural stem cell differentiation.
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DOI：
10.1016/j.biomaterials.2013.07.101
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发表时间：
2013-11
期刊：
影响因子：
14
通讯作者：
Choi, Jeong-Woo
中科院分区：
文献类型：
--
作者：
Kim, Tae-Hyung;Lee, Ki-Bum;Choi, Jeong-Woo
关键词：
监测干细胞分化和多能性是干细胞在再生医学领域实际应用的重要步骤。因此，非常需要一种能够原位监测干细胞分化的新的非破坏性检测工具。在这项研究中，我们报告了一个三维氧化石墨烯封装的金纳米粒子，这是非常有效的检测神经干细胞（NSCs）的分化潜力的基础上表面增强拉曼光谱（SERS）。开发了一种新的材料，3D GO封装的金纳米颗粒，以诱导氧化石墨烯和金纳米颗粒对Sers信号的双重增强效应，该效应仅对未分化的NSCs有效。从氧化石墨烯（GO）包封的金纳米颗粒上的未分化的NSC获得的拉曼峰比从正常金属结构获得的峰高3.5倍，并且与分化的细胞的峰明显区分开。发现C=C键的数目与1656 cm-1处的拉曼强度呈正相关，这与神经干细胞的分化状态相匹配。此外，由3D GO封装的金纳米颗粒组成的基底也可以通过电化学和电学技术有效地区分单个NSC的分化状态。因此，所提出的技术可以用作一个强大的非破坏性原位监测工具，用于识别各种干细胞（间充质干细胞，造血干细胞和神经干细胞）的分化潜力。
Monitoring of stem cell differentiation and pluripotency is an important step for the practical use of stem cells in the field of regenerative medicine. Hence, a new non-destructive detection tool capable of in situ monitoring of stem cell differentiation is highly needed. In this study, we report a 3D graphene oxide-encapsulated gold nanoparticle that is very effective for the detection of the differentiation potential of neural stem cells (NSCs) based on surface-enhanced Raman spectroscopy (SERS). A new material, 3D GO-encapsulated gold nanoparticle, is developed to induce the double enhancement effect of graphene oxide and gold nanoparticle on SERS signals which is only effective for undifferentiated NSCs. The Raman peaks achieved from undifferentiated NSCs on the graphene oxide (GO)-encapsulated gold nanoparticles were 3.5 times higher than peaks obtained from normal metal structures and were clearly distinguishable from those of differentiated cells. The number of C=C bonds and the raman instensity at 1656cm−1 was found to show a positive correlation, which matches the differentiation state of the NSCs. Moreover, the substrate composed of 3D GO-encapsulated gold nanoparticles was also effective at distinguishing the differentiation state of single NSC by using electrochemical and electrical techniques. Hence, the proposed technique can be used as a powerful non-destructive in situ monitoring tool for the identification of the differentiation potential of various kinds of stem cells (mesenchymal, hematopoietic, and neural stem cells).
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影响因子：
64.8
作者：
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作者：
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通讯作者：
Choi, Jeong-Woo
影响因子：
3.8
作者：
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通讯作者：
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