Expression profiling of sexually dimorphic genes in the Japanese quail, Coturnix japonica.
Expression profiling of sexually dimorphic genes in the Japanese quail, Coturnix japonica.
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DOI：
10.1038/s41598-020-77094-y
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发表时间：
2020-11-30
影响因子：
4.6
通讯作者：
Kuroiwa A
中科院分区：
文献类型：
--
作者：
Okuno M;Miyamoto S;Itoh T;Seki M;Suzuki Y;Mizushima S;Kuroiwa A
鸟类性别决定的研究主要集中在鸡身上。在这项研究中，我们建立了另一个广泛使用的动物模型，日本鹌鹑（Coturnix japonica）的效用，阐明性腺性别分化的分子机制。特别地，我们通过mRNA-seq对三个阶段（HH 27、HH 31和HH 38）的胚胎性腺进行了全面的基因表达谱分析。我们将4，815个基因的表达模式根据阶段之间的变化程度分为9个簇。聚类2（特征为初始增加和稳定的水平之后），包括495和310个基因表达的男性和女性，分别含有5个关键基因参与性腺性别分化。GO分析表明，该基因簇中的基因与生殖结构发育等发育过程相关，生殖发育过程中的相关基因显著，表明表达谱分析是筛选新候选基因的有效方法。基于RNA-seq数据和原位杂交，大多数性腺性别分化关键基因的表达模式和定位与鸡的表达模式和定位相一致。我们的研究结果支持日本鹌鹑作为研究鸟类性腺性别分化的模型的有效性。
Research on avian sex determination has focused on the chicken. In this study, we established the utility of another widely used animal model, the Japanese quail (Coturnix japonica), for clarifying the molecular mechanisms underlying gonadal sex differentiation. In particular, we performed comprehensive gene expression profiling of embryonic gonads at three stages (HH27, HH31 and HH38) by mRNA-seq. We classified the expression patterns of 4,815 genes into nine clusters according to the extent of change between stages. Cluster 2 (characterized by an initial increase and steady levels thereafter), including 495 and 310 genes expressed in males and females, respectively, contained five key genes involved in gonadal sex differentiation. A GO analysis showed that genes in this cluster are related to developmental processes including reproductive structure development and developmental processes involved in reproduction were significant, suggesting that expression profiling is an effective approach to identify novel candidate genes. Based on RNA-seq data and in situ hybridization, the expression patterns and localization of most key genes for gonadal sex differentiation corresponded well to those of the chicken. Our results support the effectiveness of the Japanese quail as a model for studies gonadal sex differentiation in birds.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
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作者：
Ainsworth, Sophie J.;Stanley, Rachael L.;Evans, Darrell J. R.
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影响因子：
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作者：
Arango, NA;Lovell-Badge, R;Behringer, RR
通讯作者：
Behringer, RR
影响因子：
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作者：
Hirst, Claire E.;Major, Andrew T.;Smith, Craig A.
通讯作者：
Smith, Craig A.
影响因子：
56.9
作者：
ELBRECHT, A;SMITH, RG
通讯作者：
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