Comparative study of DAM, Dof, and WRKY gene families in fourteen species and their expression in Vitis vinifera
Comparative study of DAM, Dof, and WRKY gene families in fourteen species and their expression in Vitis vinifera
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十四种葡萄中 DAM、Dof、WRKY 基因家族及其表达的比较研究
DOI：
10.1007/s13205-019-2039-3
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发表时间：
2020-01
期刊：
影响因子：
2.8
通讯作者：
Fang Jinggui
中科院分区：
文献类型：
--
作者：
Shangguan Lingfei;Chen Mengxia;Fang Xiang;Xie Zhenqiang;Zhang Kekun;Zheng Ting;Pu Yunfeng;Fang Jinggui
花蕾休眠是植物抵御严寒胁迫的重要防御机制之一，DAM、DOF和WRKY参与了包括花蕾休眠在内的许多生物学过程。本研究分析了葡萄(Vitis Vinifera)等13种植物(6种木本植物和7种草本植物)DAM、DOF和WRKY基因家族成员的数量、序列结构和进化模式。此外，还研究了VvDAM、VvDof和VvWRKY基因的表达。因此，从所选基因组中分离到51DAM、1,205WRKY和489Dof3个基因，而在10个基因组中发现了5DAM、114WRKY和50Dof重复基因对。此外，WGD和节段性重复事件与DOF和WRKY基因家族的大部分扩增相关。在花蕾休眠期间差异表达的VvDAM、VvDof和VvWRKY基因显著多于在果实发育和非生物胁迫下显著差异表达的基因。有趣的是，已鉴定出多个胁迫响应基因，如VvDAM(Vit_00s0313g00070)、两个VvDofgene(Vit_18s0001g11310和Vit_02s0025g02250)和两个VvWRKY基因(Vit_07s0031g01710和Vit_11s0052g00450)。这些数据为研究芽休眠和对非生物胁迫(即盐、干旱、铜和内涝)的响应的分子生物学研究提供了候选基因。
Bud dormancy is one of the most important defensive mechanisms through which plants resist cold stress during harsh winter weather.DAM,Dof, andWRKYhave been reported to be involved in many biological processes, including bud dormancy. In the present study, grapevine (Vitis vinifera) and other thirteen plants (six woody plants and seven herbaceous plants) were analyzed for the quantity, sequence structure, and evolution patterns of theirDAM,Dof, andWRKYgene family members. Moreover, the expression ofVvDAM,VvDof, andVvWRKYgenes was also investigated. Thus, 51DAM, 1,205WRKY, and 489Dofgenes were isolated from selected genomes, while 5DAM, 114WRKY, and 50Dofduplicate gene pairs were identified in 10 genomes. Moreover, WGD and segmental duplication events were associated with the majority of the expansions ofDofandWRKYgene families. TheVvDAM,VvDof, andVvWRKYgenes significantly differentially expressed throughout bud dormancy outnumbered those significantly differentially expressed throughout fruit development or under abiotic stresses. Interestingly, multiple stress responsive genes were identified, such asVvDAM(VIT_00s0313g00070), twoVvDofgenes (VIT_18s0001g11310 and VIT_02s0025g02250), and twoVvWRKYgenes (VIT_07s0031g01710 and VIT_11s0052g00450). These data provide candidate genes for molecular biology research investigating bud dormancy and responses to abiotic stresses (namely salt, drought, copper, and waterlogging).
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影响因子：
11.6
作者：
Ruttink, Tom;Arend, Matthias;Rohde, Antje
通讯作者：
Rohde, Antje
影响因子：
5.6
作者：
Song H;Wang P;Lin JY;Zhao C;Bi Y;Wang X
通讯作者：
Wang X
Pyrus bretschneideri 中 Dof 家族的特征及 PbDof9.2 在开花时间调控中的作用
影响因子：
4.4
作者：
Liu, Xueying;Liu, Zhe;Wang, Peng
通讯作者：
Wang, Peng
影响因子：
4.4
作者：
Ma J;Li MY;Wang F;Tang J;Xiong AS
通讯作者：
Xiong AS
DOI：
--
发表时间：
2014
期刊：
--
影响因子：
--
作者：
G. Ríos;A. Conejero;C. Leida;C. Petri;L. Burgos;M. Badenes
通讯作者：
G. Ríos;A. Conejero;C. Leida;C. Petri;L. Burgos;M. Badenes