Spinster Controls Dpp Signaling during Glial Migration in the Drosophila Eye
Spinster Controls Dpp Signaling during Glial Migration in the Drosophila Eye
复制标题
老处女控制果蝇眼睛神经胶质细胞迁移过程中的 Dpp 信号传导
作者：
Yeliz Yuva;Ann;C. Klämbt
多细胞生物的发展需要多种细胞类型的良好平衡和协调的迁移。这在发育中的神经系统中尤为重要，因为神经胶质细胞经常要移动很长的距离才能到达它们的目标。果蝇胚胎周围神经系统的大部分胶质细胞来源于中枢神经系统，并沿运动轴突向目标移动。在发育中的果蝇眼中，中枢神经系统来源的神经胶质细胞向新生的光感受器细胞外移动，但神经胶质细胞迁移与眼视盘生长的分子机制尚不清楚。在这里，我们使用增强子陷阱方法来鉴定基因spinster，该基因编码参与内核溶酶体运输的多通跨膜蛋白，在许多胶质细胞中表达。老处女突变体的特征是神经胶质过度迁移。基因实验表明，Spinster调节了几个信号级联的活动。在迁移的神经周围胶质细胞中，Spinster需要下调Dpp （Decapentaplegic）信号活性，从而停止迁移能力。此外，Spinster影响地毯细胞的生长，从而间接调节神经胶质的迁移。
The development of multicellular organisms requires the well balanced and coordinated migration of many cell types. This is of particular importance within the developing nervous system, where glial cells often move long distances to reach their targets. The majority of glial cells in the peripheral nervous system of the Drosophila embryo is derived from the CNS and migrates along motor axons toward their targets. In the developing Drosophila eye, CNS-derived glial cells move outward toward the nascent photoreceptor cells, but the molecular mechanisms coupling the migration of glial cells with the growth of the eye imaginal disc are mostly unknown. Here, we used an enhancer trap approach to identify the gene spinster, which encodes a multipass transmembrane protein involved in endosome–lysosome trafficking, as being expressed in many glial cells. spinster mutants are characterized by glial overmigration. Genetic experiments demonstrate that Spinster modulates the activity of several signaling cascades. Within the migrating perineurial glial cells, Spinster is required to downregulate Dpp (Decapentaplegic) signaling activity, which ceases migratory abilities. In addition, Spinster affects the growth of the carpet cell, which indirectly modulates glial migration.
使用无七嵌合体的辣根过氧化物酶新娘对果蝇内吞运输进行遗传解剖：钩子是多泡核内体正常成熟所必需的。
影响因子：
3.3
作者：
Sunio,A;Metcalf,AB;Krämer,H
通讯作者：
Krämer,H
影响因子：
4.6
作者：
J. Treisman;Gerald M. Rubin
通讯作者：
J. Treisman;Gerald M. Rubin