Genetic dissection of endocytic trafficking in Drosophila using a horseradish peroxidase-bride of sevenless chimera: hook is required for normal maturation of multivesicular endosomes.
Genetic dissection of endocytic trafficking in Drosophila using a horseradish peroxidase-bride of sevenless chimera: hook is required for normal maturation of multivesicular endosomes.
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使用无七嵌合体的辣根过氧化物酶新娘对果蝇内吞运输进行遗传解剖：钩子是多泡核内体正常成熟所必需的。
DOI：
10.1091/mbc.10.4.847
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发表时间：
1999
影响因子：
3.3
通讯作者：
Krämer,H
中科院分区：
文献类型：
--
作者：
Sunio,A;Metcalf,AB;Krämer,H
hook基因突变改变果蝇内化配体的胞内运输为了更详细地剖析这种缺陷，我们开发了一种新的方法来可视化七少新娘（Boss）配体内化到R7细胞后所采取的途径。在R8细胞中表达由HRP与Boss融合组成的嵌合蛋白（HRP-Boss）。这种嵌合体是完全功能性的：它拯救了bossmutant表型，并且它的运输与野生型Boss蛋白的运输没有区别。嵌合体的HRP活性用于在超微结构水平上追踪HRP-Boss通过R7细胞中的早期和晚期内体的运输。在野生型和hook突变眼盘中，HRP-Boss被内化到R7细胞中。在野生型组织中，Boss在R7细胞内的成熟多泡体（MVB）中积累;然而，在鹰眼盘中未观察到这种积累。定量电子显微镜检查显示，与野生型相比，hookmutant组织中成熟的MVB丢失，而检测到的多片层晚期内体数量是野生型的两倍多。我们的遗传分析表明，钩是所需的内吞运输后期负调控交付成熟的MVB到multilammelar晚期内体和溶酶体。
Mutations in thehookgene alter intracellular trafficking of internalized ligands inDrosophila. To dissect this defect in more detail, we developed a new approach to visualize the pathway taken by the Bride of Sevenless (Boss) ligand after its internalization into R7 cells. A chimeric protein consisting of HRP fused to Boss (HRP-Boss) was expressed in R8 cells. This chimera was fully functional: it rescued thebossmutant phenotype, and its trafficking was indistinguishable from that of the wild-type Boss protein. The HRP activity of the chimera was used to follow HRP-Boss trafficking on the ultrastructural level through early and late endosomes in R7 cells. In both wild-type andhookmutant eye disks, HRP-Boss was internalized into R7 cells. In wild-type tissue, Boss accumulated in mature multivesicular bodies (MVBs) within R7 cells; such accumulation was not observed inhookeye disks, however. Quantitative electron microscopy revealed a loss of mature MVBs inhookmutant tissue compared with wild type, whereas more than twice as many multilammelar late endosomes were detected. Our genetic analysis indicates that Hook is required late in endocytic trafficking to negatively regulate delivery from mature MVBs to multilammelar late endosomes and lysosomes.
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DOI：
10.1146/annurev.cb.11.110195.000245
发表时间：
1995
影响因子：
11.3
作者：
J. Stack;B. Horazdovsky;S. Emr
通讯作者：
J. Stack;B. Horazdovsky;S. Emr
七少膜结合配体与七少酪氨酸激酶受体的相互作用
影响因子：
64.8
作者：
H. Krämer;Ross L. Cagan;S. L. Zipursky
通讯作者：
S. L. Zipursky
影响因子：
10.5
作者：
HART, AC;KRAMER, H;ZIPURSKY, SL
通讯作者：
ZIPURSKY, SL
使用共聚焦显微镜对低密度脂蛋白和转铁蛋白内吞分选 HEp2 细胞进行定量。
影响因子：
4
作者：
Ghosh,RN;Gelman,DL;Maxfield,FR
通讯作者：
Maxfield,FR
影响因子：
64.5
作者：
FELDER, S;MILLER, K;HOPKINS, CR
通讯作者：
HOPKINS, CR