Dietary quercetin and vitamin E supplementation modulates the reproductive performance and antioxidant capacity of aged male breeder chickens.
Dietary quercetin and vitamin E supplementation modulates the reproductive performance and antioxidant capacity of aged male breeder chickens.
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膳食槲皮素和维生素 E 补充剂可调节老年雄性种鸡的繁殖性能和抗氧化能力
DOI：
10.1016/j.psj.2022.101851
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发表时间：
2022-06
期刊：
影响因子：
4.4
通讯作者：
Zhao, Xiaoling
中科院分区：
文献类型：
--
作者：
Amevor, Felix Kwame;Cui, Zhifu;Ning, Zifan;Shu, Gang;Du, Xiaxia;Jin, Ningning;Deng, Xun;Xu, Dan;Tian, Yaofu;Zhang, Yao;Li, Diyan;Wang, Yan;Du, Xiaohui;Zhao, Xiaoling
年老的公鸡经历精子发生、抗氧化能力、免疫力和激素合成的快速下降。维生素E在动物的生殖、神经系统功能和抗病能力中起着重要作用。槲皮素还具有多种生物学作用，如抗氧化能力、免疫刺激和保护精子质膜。本研究探讨了槲皮素（Q）和维生素E（VE）联合应用对老年公鸡精子质量、抗氧化能力、免疫功能以及精子发生、免疫、细胞凋亡和炎症相关基因表达的影响。选用65周龄天府种公鸡120只，随机分为4个处理，每个处理设3个重复，每个重复10只。在基础日粮的基础上，分别饲喂含Q（0.4g/kg）、VE（0.2g/kg）、Q+VE（0.4g/kg + 0.2g/kg）的日粮，试验期11周。在实验期结束时，每个重复从2只鸡中采集血液、精液、肝脏、睾丸和脾脏样品。血清激素，抗氧化参数，细胞因子和免疫球蛋白进行了评价，并在睾丸和肝组织中确定与精子发生，细胞凋亡和炎症相关的基因的mRNA表达。结果表明，槲皮素和维生素E联合应用可显著提高老龄公雏鸡精子数和精子活力，提高睾酮、促卵泡激素、促黄体生成素、抗氧化酶（超氧化物歧化酶、谷胱甘肽、总抗氧化能力）和血清免疫球蛋白（伊加、IgM）水平，并对肝脏损伤具有保护作用。此外，Q+VE还能显著增加精子发生相关基因AR、pgk 2、Cyclin A1和Cyclin A2、免疫相关基因IFN-γ和IL-2以及抗炎细胞因子IL-10的表达（P < 0.05），而降低促炎细胞因子IL-1β和IL-6的表达（P < 0.05）。综上所述，这些数据表明，槲皮素和维生素E的组合改善了生殖特性，如精子发生，精子质量和激素调节，以及促进抗氧化防御，肝保护能力和免疫反应，在老年公鸡没有任何不利影响。
Aged male chickens experience rapid declines in spermatogenesis, antioxidant capacity, immunity, and hormone synthesis. Vitamin E plays a significant role in reproduction, nervous system function, and disease resistance in animals. Quercetin also exerts many biological effects, such as antioxidant ability, immunostimulation, and protection of spermatozoal plasma membranes. This study evaluated the effects of combining dietary quercetin (Q) and vitamin E (VE) on sperm quality, antioxidant capacity, immunity, and expression of genes related to spermatogenesis, immunity, apoptosis, and inflammation in aged male chickens. A total of 120 Tianfu breeder male chickens (65 wk old) were randomly allotted to 4 treatments with 3 replicates (10 birds each). The birds were fed diets containing Q (0.4g/kg), VE (0.2g/kg), Q+VE (0.4g/kg + 0.2g/kg), and a basal diet for 11 wk. At the end of the experimental period, blood, semen, liver, testes, and spleen samples were collected from 2 birds per replicate. Serum hormones, antioxidant parameters, cytokines, and immunoglobulins were evaluated; and the mRNA expression of genes related to spermatogenesis, apoptosis, and inflammation are determined in the testes and liver tissues. The results showed that the combination quercetin and vitamin E significantly promoted the sperm count and motility, as well as elevated the levels of testosterone, follicle-stimulating hormone, and luteinizing hormone, antioxidant enzymes (Superoxide dismutase, Glutathione, and Total antioxidant capacity), and serum immunoglobulins (IgA and IgM) in the aged male chickens; also Q+VE showed protective effects on the liver against injury. In addition, Q+VE significantly increased the expression of genes related to spermatogenesis (AR, pgk2, Cyclin A1, and Cyclin A2), immunity (IFN-γ and IL-2), and anti-inflammatory cytokines (IL-10) (P < 0.05), whereas the expression of proinflammatory cytokines (IL-1β and IL-6) was decreased (P < 0.05). Taken together, these data indicate that the combination of quercetin and vitamin E improved reproductive characteristics such as spermatogenesis, sperm quality, and hormone regulation, as well as promoted antioxidant defense, hepatoprotective capacity, and immune response in aged male chickens without any detrimental effects.
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影响因子：
2.4
作者：
Jeong SM;Kang MJ;Choi HN;Kim JH;Kim JI
通讯作者：
Kim JI
影响因子：
6.5
作者：
Bubnov, Rostyslav V.;Drahulian, Maria V.;Gulko, Tamara P.
通讯作者：
Gulko, Tamara P.
影响因子：
2.8
作者：
Biswas, A.;Mohan, J.;Tyagi, J. S.
通讯作者：
Tyagi, J. S.
DOI：
10.18632/aging.100531
发表时间：
2013-01
期刊：
Aging
影响因子：
--
作者：
Howcroft TK;Campisi J;Louis GB;Smith MT;Wise B;Wyss-Coray T;Augustine AD;McElhaney JE;Kohanski R;Sierra F
通讯作者：
Sierra F
影响因子：
3.6
作者：
Arango, Nelson Alexander;Li, Li;Donahoe, Patricia K.
通讯作者：
Donahoe, Patricia K.