Hemin binding by Porphyromonas gingivalis strains is dependent on the presence of A-LPS.
Hemin binding by Porphyromonas gingivalis strains is dependent on the presence of A-LPS.
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DOI：
10.1111/omi.12178
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发表时间：
2017-10
影响因子：
3.7
通讯作者：
Curtis MA
中科院分区：
文献类型：
--
作者：
Rangarajan M;Aduse-Opoku J;Paramonov NA;Hashim A;Curtis MA
牙龈卟啉单胞菌是一种革兰氏阴性的黑色色素厌氧菌，不能合成血红素[Fe（II）‐原卟啉IX]或血红素[Fe（III）‐原卟啉IX‐Cl]，这是重要的生长/毒力因子，因此必须从宿主体内获得。牙龈卟啉单胞菌表达几种蛋白血红素结合位点，这些位点在宿主血红素/血红素的结合/运输中起重要作用。它还合成了几种毒力因子，即半胱氨酸蛋白酶Arg -和Lys - gingipains和两种脂多糖（LPS）， O - LPS和A - LPS。牙龈炎是产生黑色色素μ‐oxo‐bisheme {[Fe(III)PPIX] 2o}所必需的，该色素来源于血红蛋白并沉积在细菌细胞表面，当生长在血琼脂上时导致典型的黑色菌落。在这项研究中，我们研究了LPS在细胞表面沉积μ -氧- bisheme中的作用。一个在精氨酸- gingipain生物合成上有缺陷的牙龈卟啉突变体，即rgpA/rgpB，在血琼脂上产生棕色菌落，而在赖氨酸- gingipain （kgp）和脂多糖生物合成上有缺陷的突变体，即porR、waaL、wzy和pg0129 （α‐1,3‐甘露糖基转移酶）产生非色素菌落。然而，只有那些缺乏A‐LPS的突变体在悬浮细胞与血红蛋白孵育时显示出血红蛋白结合减少。使用原生、去0磷酸化和去脂化的牙龈假单胞菌W50和porR菌株的LPS，我们证明与与A - PS的结合相比，血红蛋白与O -多糖（PS）和脂质A部分的结合减少了。我们得出结论，牙龈卟啉卟啉外膜中的A‐LPS作为支架/锚定物，在细胞表面保留μ‐氧化素，并且色素沉着依赖于A‐LPS的存在。
Porphyromonas gingivalis is a Gram‐negative black pigmenting anaerobe that is unable to synthesize heme [Fe(II)‐protoporphyrin IX] or hemin [Fe(III)‐protoporphyrin IX‐Cl], which are important growth/virulence factors, and must therefore derive them from the host. Porphyromonas gingivalis expresses several proteinaceous hemin‐binding sites, which are important in the binding/transport of heme/hemin from the host. It also synthesizes several virulence factors, namely cysteine‐proteases Arg‐ and Lys‐gingipains and two lipopolysaccharides (LPS), O‐LPS and A‐LPS. The gingipains are required for the production of the black pigment, μ‐oxo‐bisheme {[Fe(III)PPIX]2 O}, which is derived from hemoglobin and deposited on the bacterial cell‐surface leading to the characteristic black colonies when grown on blood agar. In this study we investigated the role of LPS in the deposition of μ‐oxo‐bisheme on the cell‐surface. A P. gingivalis mutant defective in the biosynthesis of Arg‐gingipains, namely rgpA/rgpB, produces brown colonies on blood agar and mutants defective in Lys‐gingipain (kgp) and LPS biosynthesis namely porR, waaL, wzy, and pg0129 (α‐1, 3‐mannosyltransferase) produce non‐pigmented colonies. However, only those mutants lacking A‐LPS showed reduced hemin‐binding when cells in suspension were incubated with hemin. Using native, de‐O‐phosphorylated and de‐lipidated LPS from P. gingivalis W50 and porR strains, we demonstrated that hemin‐binding to O‐polysaccharide (PS) and to the lipid A moiety of LPS was reduced compared with hemin‐binding to A‐PS. We conclude that A‐LPS in the outer‐membrane of P. gingivalis serves as a scaffold/anchor for the retention of μ‐oxo‐bisheme on the cell surface and pigmentation is dependent on the presence of A‐LPS.
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影响因子：
4.2
作者：
Shoji M;Shibata Y;Shiroza T;Yukitake H;Peng B;Chen YY;Sato K;Naito M;Abiko Y;Reynolds EC;Nakayama K
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Nakayama K
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通讯作者：
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