Lactose in human breast milk an inducer of innate immunity with implications for a role in intestinal homeostasis.
Lactose in human breast milk an inducer of innate immunity with implications for a role in intestinal homeostasis.
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DOI：
10.1371/journal.pone.0053876
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Agerberth B
中科院分区：
文献类型：
--
作者：
Cederlund A;Kai-Larsen Y;Printz G;Yoshio H;Alvelius G;Lagercrantz H;Strömberg R;Jörnvall H;Gudmundsson GH;Agerberth B
产后，婴儿尚未建立起功能齐全的适应性免疫系统，有感染的风险。因此，新生儿依赖于先天免疫系统，其抗菌肽（AMP）和蛋白质在上皮表面表达。母乳中有几种因素可以提供免疫保护，但哪些决定性因素以及它们的工作方式尚不清楚。在这里，我们从人乳中分离出一种AMP诱导因子，并通过电喷雾质谱和NMR鉴定为乳糖。它以剂量和时间依赖性方式诱导结肠上皮细胞中编码唯一的人凯萨林菌素LL-37的基因（CAMP）。两种不同的p38拮抗剂抑制诱导，表明通过p38依赖性途径的影响。乳糖还诱导结肠上皮细胞系T84和THP-1单核细胞和巨噬细胞中的cAMP。它还表现出与丁酸盐和苯丁酸盐对cAMP诱导的协同作用。总之，这些结果表明乳糖通过上调胃肠道AMP在先天免疫中的额外功能，这可能导致保护新生儿肠道免受病原体的侵害并调节婴儿的微生物群。
Postpartum, infants have not yet established a fully functional adaptive immune system and are at risk of acquiring infections. Hence, newborns are dependent on the innate immune system with its antimicrobial peptides (AMPs) and proteins expressed at epithelial surfaces. Several factors in breast milk are known to confer immune protection, but which the decisive factors are and through which manner they work is unknown. Here, we isolated an AMP-inducing factor from human milk and identified it by electrospray mass spectrometry and NMR to be lactose. It induces the gene (CAMP) that encodes the only human cathelicidin LL-37 in colonic epithelial cells in a dose- and time-dependent manner. The induction was suppressed by two different p38 antagonists, indicating an effect via the p38-dependent pathway. Lactose also induced CAMP in the colonic epithelial cell line T84 and in THP-1 monocytes and macrophages. It further exhibited a synergistic effect with butyrate and phenylbutyrate on CAMP induction. Together, these results suggest an additional function of lactose in innate immunity by upregulating gastrointestinal AMPs that may lead to protection of the neonatal gut against pathogens and regulation of the microbiota of the infant.
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影响因子：
168.9
作者：
Black, RE;Morris, SS;Bryce, J
通讯作者：
Bryce, J
影响因子：
4.2
作者：
He, T;Priebe, MG;Vonk, RJ
通讯作者：
Vonk, RJ
DOI：
10.1097/00005176-200009000-00017
发表时间：
2000-09-01
影响因子：
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作者：
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通讯作者：
Gudmundsson, GH
影响因子：
3.6
作者：
Kuntz, Sabine;Rudloff, Silvia;Kunz, Clemens
通讯作者：
Kunz, Clemens