Fibroblasts as host cells in latent leishmaniosis.
Fibroblasts as host cells in latent leishmaniosis.
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DOI：
10.1084/jem.191.12.2121
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发表时间：
2000-06-19
期刊：
影响因子：
--
通讯作者：
Rittig MG
中科院分区：
文献类型：
--
作者：
Bogdan C;Donhauser N;Döring R;Röllinghoff M;Diefenbach A;Rittig MG
已知细胞内寄生虫在疾病临床治愈后在哺乳动物宿主中终身存在，但对持续存在的机制知之甚少。在这里，我们通过共聚焦激光显微镜显示，在引流淋巴结的小鼠，治愈了皮肤感染与利什曼原虫主要，40%的持续寄生虫与成纤维细胞形成的网状淋巴结。在离体培养条件下，L.主要感染原发性皮肤或淋巴结成纤维细胞。与巨噬细胞相比，苦参碱激活的成纤维细胞表达2型一氧化氮合酶和杀伤细胞内L。少校这些数据确定成纤维细胞作为一个重要的宿主细胞利什曼原虫在慢性感染阶段，并建议他们可能作为安全的目标，寄生虫在临床潜伏性疾病。
Intracellular parasites are known to persist lifelong in mammalian hosts after the clinical cure of the disease, but the mechanisms of persistence are poorly understood. Here, we show by confocal laser microscopy that in the draining lymph nodes of mice that had healed a cutaneous infection with Leishmania major, 40% of the persisting parasites were associated with fibroblasts forming the reticular meshwork of the lymph nodes. In vitro, both promastigotes and amastigotes of L. major infected primary skin or lymph node fibroblasts. Compared with macrophages, cytokine-activated fibroblasts had a reduced ability to express type 2 nitric oxide synthase and to kill intracellular L. major. These data identify fibroblasts as an important host cell for Leishmania during the chronic phase of infection and suggest that they might serve as safe targets for the parasites in clinically latent disease.
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DOI：
10.1083/jcb.123.3.759
发表时间：
1993-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Love DC;Esko JD;Mosser DM
通讯作者：
Mosser DM
DOI：
10.1016/s0035-9203(40)90043-8
发表时间：
1940-01-01
期刊：
TRANS ROY SOC TROP MED AND HYG
影响因子：
--
作者：
ADLER, S.
通讯作者：
ADLER, S.
影响因子：
56.9
作者：
Diefenbach, A;Schindler, H;Bogdan, C
通讯作者：
Bogdan, C
影响因子：
5.4
作者：
MOLL, H;FLOHE, S;ROLLINGHOFF, M
通讯作者：
ROLLINGHOFF, M
DOI：
10.1016/s0169-4758(96)40010-2
发表时间：
1997-02-01
期刊：
PARASITOLOGY TODAY
影响因子：
--
作者：
Lima, HC;Bleyenberg, JA;Titus, RG
通讯作者：
Titus, RG