Recovery from volumetric muscle loss injury: A comparison between young and aged rats.
Recovery from volumetric muscle loss injury: A comparison between young and aged rats.
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DOI：
10.1016/j.exger.2016.07.008
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发表时间：
2016-10
影响因子：
3.9
通讯作者：
Wolchok, Jeffrey C.
中科院分区：
文献类型：
--
作者：
Kim, John T.;Kasukonis, Benjamin M.;Brown, Lemuel A.;Washington, Tyrone A.;Wolchok, Jeffrey C.
肌肉体积损失（VML），骨骼肌组织的大量损失，无论是通过创伤或手术，破坏了修复的能力，导致非收缩性疤痕组织的形成。生肌潜能，沿着影响伤口修复的其他因素，已知随着年龄的增长而下降。为了制定有效的治疗策略，VML损伤是有效的，在广泛的患者人群，有必要了解如何响应VML损伤是受老化。为此，本研究旨在比较年轻和老年动物组对下肢VML损伤的反应。年轻（3个月，n=12）和老年（18个月，n=8）雄性Fischer 344大鼠进行手术胫骨前肌VML损伤。在VML损伤后三个月，发现当未进行VML损伤时，年轻TA肌肉平均比老年肌肉重16%，当比较VML治疗的年轻和老年动物时，年轻TA肌肉平均比老年肌肉重25%（p<0.0001，p<0.0001）。年轻组和老年组的峰值收缩力在VML损伤后显著降低，分别产生对侧肢体峰值力的65%和59%（p<0.0001）。然而，没有发现基于年龄的峰值收缩力的差异，表明VML影响肌肉的修复能力，无论年龄如何。在这项研究中，我们使用胶原蛋白I与MyoD表达的比率作为纤维化与肌生成的指标。随着年龄的增长，纤维横截面积减少（p<0.005），再加上胶原蛋白I与MyoD表达的比率（随年龄增加），支持老年人群中再生受损有利于纤维化的观点（p=0.0241）。VML损伤也加剧了这种损伤，在老年组中观察到胶原蛋白I与MyoD的比例增加了77倍（p<0.0002）。本研究中描述的老年动物模型为研究人员提供了一种工具，不仅可以探索VML损伤策略的发展，还可以探索衰老对肌肉再生的影响。
Termed volumetric muscle loss (VML), the bulk loss of skeletal muscle tissue either through trauma or surgery overwhelms the capacity for repair, leading to the formation of non-contractile scar tissue. The myogenic potential, along with other factors that influence wound repair are known to decline with age. In order to develop effective treatment strategies for VML injuries that are effective across a broad range of patient populations, it is necessary to understand how the response to VML injury is affected by aging. Towards this end, this study was conducted to compare the response of young and aged animal groups to a lower extremity VML injury. Young (3 months, n=12) and aged (18 months, n=8) male Fischer 344 rats underwent surgical VML injury of the tibialis anterior muscle. Three months after VML injury it was found that young TA muscle was on average 16% heavier than aged muscle when no VML injury was performed and 25% heavier when comparing VML treated young and aged animals (p<0.0001, p<0.0001). Peak contractile force for both the young and aged groups was found to decrease significantly following VML injury, producing 65% and 59% of the contralateral limbs’ peak force, respectively (p<0.0001). However, there were no differences found for peak contractile force based on age, suggesting that VML affects muscle’s ability to repair, regardless of age. In this study, we used the ratio of collagen I to MyoD expression as a metric for fibrosis vs. myogenesis. Decreasing fiber cross-sectional area with advancing age (p<0.005) coupled with the ratio of collagen I to MyoD expression, which increased with age, supports the thought that regeneration is impaired in the aged population in favor of fibrosis (p=0.0241). This impairment is also exacerbated by the contribution of VML injury, where a 77-fold increase in the ratio of collagen I to MyoD was observed in the aged group (p<0.0002). The aged animal model described in this study provides a tool for investigators exploring not only the development of VML injury strategies but also the effect of aging on muscle regeneration.
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影响因子：
3.3
作者：
Barani, AE;Durieux, AC;Freyssenet, D
通讯作者：
Freyssenet, D
影响因子：
3.3
作者：
Garg, Koyal;Corona, Benjamin T.;Walters, Thomas J.
通讯作者：
Walters, Thomas J.
影响因子：
2.8
作者：
GOLDSPINK, G;FERNANDES, K;WELLS, DJ
通讯作者：
WELLS, DJ
影响因子：
6.3
作者：
Brown, L. A.;Lee, D. E.;Washington, T. A.
通讯作者：
Washington, T. A.
DOI：
10.1186/2052-1847-6-41
发表时间：
2014
期刊：
BMC sports science, medicine & rehabilitation
影响因子：
--
作者：
Aurora A;Garg K;Corona BT;Walters TJ
通讯作者：
Walters TJ