Clinical relevance of microRNA miR-21, miR-31, miR-92a, miR-101, miR-106a and miR-145 in colorectal cancer.
Clinical relevance of microRNA miR-21, miR-31, miR-92a, miR-101, miR-106a and miR-145 in colorectal cancer.
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DOI：
10.1186/1471-2407-12-505
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发表时间：
2012-11-02
期刊：
影响因子：
3.8
通讯作者：
Flatmark K
中科院分区：
文献类型：
--
作者：
Schee K;Boye K;Abrahamsen TW;Fodstad Ø;Flatmark K
MicroRNAs(MiRNAs)通过与mRNA结合来调节基因的表达，根据靶点的不同，可以作为癌基因或肿瘤抑制因子发挥作用。在本研究中，我们利用qRT-PCR技术检测了6种miRNAs(miR-21、miR-31、miR-92a、miR-101、miR-106a和miR-145)在193例结直肠癌患者肿瘤中的表达，并分析其与临床病理参数和患者预后的关系。MiRNAs是根据先前的研究选择的，因为它们具有生物标志物的潜力，并提示与结直肠癌的生物学相关性。用qRT-PCR检测miRNA的表达。用Mann-Whitney U和Kruskal-Wallis检验探讨miRNA表达与临床病理变量之间的关系，用Kaplan-Meier方法估计生存率，用对数等级检验进行比较。MiR-101在肿瘤标本中几乎不表达，而对于其他miRNAs，表达水平和表达范围都不同，其中miR-21相对于参考(RNU44)的表达最为丰富。在我们的研究队列中，只有miR-31具有重要的临床意义，因为高表达与肿瘤分期晚期和低分化相关。所研究的miRNAs的表达与无转移或总生存期之间没有发现明显的相关性。研究了六个先前被确定为结直肠癌候选生物标记物的miRNAs的表达，在我们的患者队列中几乎没有检测到临床相关的关联。我们的结果强调了在独立的患者队列中验证潜在的肿瘤标记物的重要性，并表明miRNAs作为结直肠癌生物标记物的作用仍未确定。
MicroRNAs (miRNAs) regulate gene expression by binding to mRNA, and can function as oncogenes or tumor suppressors depending on the target. In this study, using qRT-PCR, we examined the expression of six miRNAs (miR-21, miR-31, miR-92a, miR-101, miR-106a and miR-145) in tumors from 193 prospectively recruited patients with colorectal cancer, and associations with clinicopathological parameters and patient outcome were analyzed. The miRNAs were chosen based on previous studies for their biomarker potential and suggested biological relevance in colorectal cancer. The miRNA expression was examined by qRT-PCR. Associations between miRNA expression and clinicopathological variables were explored using Mann–Whitney U and Kruskal-Wallis test while survival was estimated using the Kaplan-Meier method and compared using the log-rank test. MiR-101 was hardly expressed in the tumor samples, while for the other miRNAs, variable expression levels and expression ranges were observed, with miR-21 being most abundantly expressed relative to the reference (RNU44). In our study cohort, major clinical significance was demonstrated only for miR-31, as high expression was associated with advanced tumor stage and poor differentiation. No significant associations were found between expression of the investigated miRNAs and metastasis-free or overall survival. Investigating the expression of six miRNAs previously identified as candidate biomarkers in colorectal cancer, few clinically relevant associations were detected in our patient cohort. Our results emphasize the importance of validating potential tumor markers in independent patient cohorts, and indicate that the role of miRNAs as colorectal cancer biomarkers is still undetermined.
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